Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a potential target for antimicrobial drug design, i.e. in the treatment of cystic fibrosis caused by Burkholderia cenocepacia | Burkholderia cenocepacia |
Cloned (Comment) | Organism |
---|---|
gene bceA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Burkholderia cenocepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
GDP-mannose | pH 7.6, recombinant enzyme, in presence of 5 mM Mg2+ and 2 mM diphosphate | Burkholderia cenocepacia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | GMP activity is strongly dependent on the presence of Ca2+ or Mn2+ | Burkholderia cenocepacia | |
Co2+ | activates slightly | Burkholderia cenocepacia | |
Mg2+ | activates | Burkholderia cenocepacia | |
Mn2+ | GMP activity is strongly dependent on the presence of Ca2+ or Mn2+, the presence of 5 mM Mn2+, precipitation occurred in the reaction mixture | Burkholderia cenocepacia | |
additional information | descending order of activation Mg2+, Ca2+, Mn2+, Co2+, Ni2+ | Burkholderia cenocepacia | |
Ni2+ | activates slightly | Burkholderia cenocepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55716 | - |
x * 56000, recombinant enzyme, SDS-PAGE, x * 55716, sequence caculation | Burkholderia cenocepacia |
56000 | - |
x * 56000, recombinant enzyme, SDS-PAGE, x * 55716, sequence caculation | Burkholderia cenocepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + GDP-mannose | Burkholderia cenocepacia | - |
GTP + alpha-D-mannose 1-phosphate | - |
? | |
additional information | Burkholderia cenocepacia | the enzyme of Burkholderia cenocepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cenocepacia | B4EMQ6 | gene bceA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity | Burkholderia cenocepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + GDP-mannose | - |
Burkholderia cenocepacia | GTP + alpha-D-mannose 1-phosphate | - |
? | |
additional information | the enzyme of Burkholderia cenocepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities | Burkholderia cenocepacia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, recombinant enzyme, SDS-PAGE, x * 55716, sequence caculation | Burkholderia cenocepacia |
More | the enzyme's amino acid sequence contains several conserved motifs: 1. the pyrophosphorylase signature sequence 38GGXGXRLXPLX5PK54, 2. the GMP active site 210FVEKP214, 3. the zinc-binding motif 436QXH438 and the putative PMI active site 465EN(Q/E)SX (Y/F)I471 | Burkholderia cenocepacia |
Synonyms | Comment | Organism |
---|---|---|
BceA(J) protein | - |
Burkholderia cenocepacia |
BceAJ | - |
Burkholderia cenocepacia |
GDP-D-mannose pyrophosphorylase | - |
Burkholderia cenocepacia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Burkholderia cenocepacia |