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Literature summary for 2.7.7.13 extracted from
- GDP-mannose pyrophosphorylase is essential for cell wall integrity, morphogenesis and viability of Aspergillus fumigatus (2008), Microbiology, 154, 2730-2739.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Afsrb1, expression in Escherichia coli strain BL21(DE3) | Aspergillus fumigatus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of the conditional inactivation mutant strain YJ-gmpp by replacement of the native Afsrb1 promoter with an inducible Aspergillus nidulans alcA promoter. Repression of GMPP in yeast leads to phenotypes including hyphal lysis, defective cell wall, and failure of polarized growth and branching site selection, and cell separation, inactivation phenotype, overview. The alkali-soluble alpha-glucan and alkali-insoluble beta-glucan contents remain unchanged in strain YJ-gmpp depleted of Afsrb1, while the alkali-soluble protein and alkali-insoluble chitin contents are 1.3 and 2.0fold higher, respectively, than those of the wild-type | Aspergillus fumigatus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Aspergillus fumigatus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aspergillus fumigatus | repression of GMPP in yeast leads to phenotypes including hyphal lysis, defective cell wall, and failure of polarized growth and branching site selection, and cell separation | ? | - |
? | |
| additional information | Aspergillus fumigatus Af293 | repression of GMPP in yeast leads to phenotypes including hyphal lysis, defective cell wall, and failure of polarized growth and branching site selection, and cell separation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | Q4U3E8 | strain YJ-407, gene Afsrb1 | - |
| Aspergillus fumigatus Af293 | Q4U3E8 | strain YJ-407, gene Afsrb1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | repression of GMPP in yeast leads to phenotypes including hyphal lysis, defective cell wall, and failure of polarized growth and branching site selection, and cell separation | Aspergillus fumigatus | ? | - |
? | |
| additional information | bifunctional protein, with phosphomannose isomerase and guanosine diphosphate-mannose pyrophosphorylase activities | Aspergillus fumigatus | ? | - |
? | |
| additional information | repression of GMPP in yeast leads to phenotypes including hyphal lysis, defective cell wall, and failure of polarized growth and branching site selection, and cell separation | Aspergillus fumigatus Af293 | ? | - |
? | |
| additional information | bifunctional protein, with phosphomannose isomerase and guanosine diphosphate-mannose pyrophosphorylase activities | Aspergillus fumigatus Af293 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDP-mannose pyrophosphorylase | - |
Aspergillus fumigatus |
| GMPP | - |
Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Aspergillus fumigatus |
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