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Literature summary for 2.7.7.15 extracted from
- The purification and characterization of CTP:phosphorylcholine cytidylyltransferase from rat liver (1986), J. Biol. Chem., 261, 5104-5110.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | - |
Rattus norvegicus |  |
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Rattus norvegicus | |
| Ca2+ | - |
Rattus norvegicus | |
| Mn2+ | - |
Rattus norvegicus | |
| additional information | no inhibition by ATP; no inhibition by CDP; no inhibition by CMP | Rattus norvegicus | |
| N-ethylmaleimide | CTP and phosphocholine protect | Rattus norvegicus | |
| phosphate | maximal inhibition of 60% at 120 mM | Rattus norvegicus | |
| Zn2+ | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
phosphorylcholine | pH 7.5 | Rattus norvegicus | |
| 0.29 | - |
CTP | pH 7.5 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
2 * 39000 + 2 * 48000, SDS-PAGE | Rattus norvegicus |
| 48000 | - |
2 * 39000 + 2 * 48000, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.25 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable in presence of Triton X-100 and 0.2 M potassium phosphate | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + choline phosphate | - |
Rattus norvegicus | diphosphate + CDPcholine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 39000 + 2 * 48000, SDS-PAGE | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | pH 6: about 80% of activity maximum, pH 8: about 60% of activity maximum | Rattus norvegicus |
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