Any feedback?
Literature summary for 2.7.7.15 extracted from
- Phosphatidylylcholine biosynthesis in castor bean endosperm. Purification and properties of cytidine 5'-triphosphate:choline-phosphate cytidylyltransferase (1990), Plant Physiol., 93, 250-255.
General Stability
| General Stability | Organism |
|---|---|
| Unstable at low protein concentration | Ricinus communis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | inhibition after preincubation with 3 mM ATP for 20 min | Ricinus communis |  |
| additional information | no inhibition by ATP | Ricinus communis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
CTP | pH 7.5 | Ricinus communis | |
| 0.37 | - |
choline phosphate | pH 7.5 | Ricinus communis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, SDS-PAGE | Ricinus communis |
| 155000 | - |
gel filtration | Ricinus communis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + choline phosphate | Ricinus communis | catalyzes a major rate-limiting step in the biosynthesis of phosphocholine | diphosphate + CDPcholine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
castor bean, L- var. Hale | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ricinus communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Ricinus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.518 | - |
- |
Ricinus communis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, enzyme concentrated to a small volume, 50% glycerol, 20% loss of activity after 2 weeks | Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + choline phosphate | - |
Ricinus communis | diphosphate + CDPcholine | - |
? | |
| CTP + choline phosphate | catalyzes a major rate-limiting step in the biosynthesis of phosphocholine | Ricinus communis | diphosphate + CDPcholine | - |
? | |
| CTP + phosphomonomethylethanolamine | - |
Ricinus communis | diphosphate + CDPmethylethanolamine | - |
? | |
| additional information | no substrates: ethanolamine phosphate and phosphodimethylethanolamine | Ricinus communis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, SDS-PAGE | Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Ricinus communis |
| 7.5 | - |
- |
Ricinus communis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
