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Literature summary for 2.7.7.15 extracted from
- Characterization of a lipid activated CTP:phosphocholine cytidylyltransferase from Drosophila melanogaster (2004), Biochim. Biophys. Acta, 1683, 78-88.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Lipids | regulation of activity by reversible association with membrane lipids, preferentially activated by anionic lipids, highest activation with phosphatidylcholine vesicles containing diphosphatidylglycerol (29fold), less activation with vesicles containing phosphatidylserine (16fold), phosphatidylinositol (21fold), phosphatidylethanolamine (17fold), and oleate (13fold), compared to free enzyme 5fold activation with enzyme associated with pure phosphatidylcholine vesicles | Drosophila melanogaster |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as native and His-tag fusion protein in Sf9 insect cells, native protein expressed in Escherichia coli but expressed protein was found to be misfolded | Drosophila melanogaster |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
choline phosphate | purified native enzyme, pH 7.0, 37°C | Drosophila melanogaster |  |
| 0.64 | - |
choline phosphate | purified His-tagged enzyme, pH 7.0, 37°C | Drosophila melanogaster | |
| 0.72 | - |
CTP | purified native enzyme, pH 7.0, 37°C | Drosophila melanogaster | |
| 0.81 | - |
CTP | purified His-tagged enzyme, pH 7.0, 37°C | Drosophila melanogaster | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | enzyme is activated when bound to vesicles | Drosophila melanogaster | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
x * 44000, SDS-PAGE | Drosophila melanogaster |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + choline phosphate | Drosophila melanogaster | rate limiting enzyme for the synthesis of phosphatidylcholine | diphosphate + CDP-choline | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylated on serine and threonine but not on tyrosine residues | Drosophila melanogaster |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and His-tag protein | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + choline phosphate | - |
Drosophila melanogaster | diphosphate + CDP-choline | - |
? | |
| CTP + choline phosphate | rate limiting enzyme for the synthesis of phosphatidylcholine | Drosophila melanogaster | diphosphate + CDP-choline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, SDS-PAGE | Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CCT2 | - |
Drosophila melanogaster |
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