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Literature summary for 2.7.7.15 extracted from
- Membrane binding modulates the quaternary structure of CTP:phosphocholine cytidylyltransferase (2004), J. Biol. Chem., 279, 28817-28825.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Lipids | regulation of activity by reversible association with membranes containing anionic lipids | Rattus norvegicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| native and mutant enzyme expressed as His-tag fusion proteins in COS-1 cells | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C113S | no significant effect of activity | Rattus norvegicus |
| C139S | no significant effect of activity | Rattus norvegicus |
| C354S | no significant effect of activity | Rattus norvegicus |
| C359S | no significant effect of activity | Rattus norvegicus |
| C37S | unable to form dimers | Rattus norvegicus |
| C68S | no significant effect of activity | Rattus norvegicus |
| C73S | no significant effect of activity | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | enzyme is activated when bound to vesicles | Rattus norvegicus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, SDS-PAGE | Rattus norvegicus |
| 80000 | 110000 | diffuse band in SDS-PAGE using chemically cross-linked protein | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + choline phosphate | Rattus norvegicus | rate limiting enzyme for the synthesis of phosphatidylcholine | diphosphate + CDP-choline | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzymes using His-tag | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + choline phosphate | - |
Rattus norvegicus | diphosphate + CDP-choline | - |
? | |
| CTP + choline phosphate | rate limiting enzyme for the synthesis of phosphatidylcholine | Rattus norvegicus | diphosphate + CDP-choline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CCTalpha | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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