Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | deamido-NAD+ as substrate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
deamido-NAD+ | 37°C, pH 7.5 | Escherichia coli | |
0.029 | - |
deamido-NAD+ | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.06 | - |
ATP | 37°C, pH 7.5, + nicotinate ribonucleotide | Saccharomyces cerevisiae | |
0.069 | - |
NAD+ | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.08 | - |
nicotinate ribonucleotide | 37°C, pH 7.5 | Escherichia coli | |
0.13 | - |
nicotinate ribonucleotide | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.2 | - |
nicotinamide ribonucleotide | 37°C, pH 7.5 | Escherichia coli | |
0.27 | - |
3-acetylpyridine-NAD+ | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.37 | - |
NAD+ | 37°C, pH 7.5 | Escherichia coli | |
0.4 | - |
nicotinamide ribonucleotide | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.5 | - |
ATP | 37°C, pH 7.5 | Escherichia coli | |
0.65 | - |
diphosphate | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.74 | - |
3-pyridinealdehyde-NAD+ | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
1.1 | - |
diphosphate | 37°C, pH 7.5 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activation, 25 mM, NAD+-synthesis, not deamido-NAD+-synthesis | Escherichia coli | |
Mg2+ | requirement | Escherichia coli | |
Mg2+ | requirement | Saccharomyces cerevisiae | |
NH4Cl | activation, can substitute for KCl | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinate ribonucleotide | Escherichia coli | - |
diphosphate + deamido-NAD+ | - |
r | |
ATP + nicotinate ribonucleotide | Saccharomyces cerevisiae | - |
diphosphate + deamido-NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Saccharomyces cerevisiae | - |
brewer's yeast | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
partial | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0003 | - |
3-acetyl-pyridine-NAD+ as substrate | Escherichia coli |
0.0008 | - |
3-pyridinealdehyde-NAD+ as substrate | Escherichia coli |
0.004 | - |
NAD+ as substrate | Escherichia coli |
0.024 | - |
deamido-NAD+ as substrate | Escherichia coli |
1.42 | - |
3-pyridinealdehyde-NAD+ as substrate | Saccharomyces cerevisiae |
3.82 | - |
3-acetyl-pyridine-NAD+ as substrate | Saccharomyces cerevisiae |
3.85 | - |
deamido-NAD+ as substrate | Saccharomyces cerevisiae |
5 | - |
NAD+ as substrate | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
0-3°C, 22% loss of activity within 31 days | Saccharomyces cerevisiae |
0-3°C, 31 days stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-acetyl-pyridine-NAD+ | poor substrate | Escherichia coli | ? | - |
? | |
ATP + 3-acetyl-pyridine-NAD+ | reaction at 76% the rate of nicotinamide ribonucleotide | Saccharomyces cerevisiae | ? | - |
? | |
ATP + 3-pyridinealdehyde-NAD+ | poor substrate | Escherichia coli | ? | - |
? | |
ATP + 3-pyridinealdehyde-NAD+ | reaction at 28% the rate of nicotinamide ribonucleotide | Saccharomyces cerevisiae | ? | - |
? | |
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide, reverse reaction at 17% the rate of deamido-NAD+-synthesis | Escherichia coli | diphosphate + NAD+ | - |
r | |
ATP + nicotinate ribonucleotide | - |
Escherichia coli | diphosphate + deamido-NAD+ | - |
r | |
ATP + nicotinate ribonucleotide | - |
Saccharomyces cerevisiae | diphosphate + deamido-NAD+ | - |
r | |
ATP + nicotinate ribonucleotide | best substrate | Escherichia coli | diphosphate + deamido-NAD+ | i.e. nicotinic acid adenine dinucleotide | r | |
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Escherichia coli | diphosphate + deamido-NAD+ | i.e. nicotinic acid adenine dinucleotide | r | |
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Saccharomyces cerevisiae | diphosphate + deamido-NAD+ | i.e. nicotinic acid adenine dinucleotide | r | |
ATP + nicotinate ribonucleotide | reaction at 77% the rate of nicotinamide ribonucleotide | Saccharomyces cerevisiae | diphosphate + deamido-NAD+ | i.e. nicotinic acid adenine dinucleotide | r | |
deoxy-ATP + nicotinamide ribonucleotide | - |
Escherichia coli | ? | - |
? | |
deoxy-ATP + nicotinamide ribonucleotide | - |
Saccharomyces cerevisiae | ? | - |
? | |
deoxy-ATP + nicotinate ribonucleotide | - |
Escherichia coli | ? | - |
? | |
deoxy-ATP + nicotinate ribonucleotide | - |
Saccharomyces cerevisiae | ? | - |
? | |
NAD+ + diphosphate | - |
Saccharomyces cerevisiae | ATP + nicotinamide nucleotide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
5 min 54% loss of activity | Escherichia coli |
60 | - |
5 min 39% loss of activity | Saccharomyces cerevisiae |
60 | - |
5 min 95% loss of activity | Escherichia coli |
65 | - |
5 min 65% loss of activity | Saccharomyces cerevisiae |