Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-dATP | - |
Mesocricetus auratus | |
CTP | - |
Mesocricetus auratus | |
diphosphate | - |
Mesocricetus auratus | |
GTP | - |
Mesocricetus auratus | |
K+ | above 50 mM; KCl | Mesocricetus auratus | |
additional information | not inhibitory: alpha-amanitin | Mesocricetus auratus | |
N-ethylmaleimide | - |
Mesocricetus auratus | |
UTP | - |
Mesocricetus auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
ATP | polymerase IIa, 37°C | Mesocricetus auratus | |
0.05 | - |
ATP | polymerase IIb, 37°C | Mesocricetus auratus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ is inactive, maximum activity in presence of both Mn2+ and Mg2+ | Mesocricetus auratus | |
Mn2+ | maximum activity in presence of both Mn2+ and Mg2+ | Mesocricetus auratus | |
Mn2+ | optimal concentration: 4 mM (polymerase IIa), 4-8 mM (polymerase IIb) | Mesocricetus auratus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 85000 + 1 * 60000, SDS-PAGE | Mesocricetus auratus |
85000 | - |
1 * 85000 + 1 * 60000, SDS-PAGE | Mesocricetus auratus |
145000 | - |
polymerase IIa, gel filtration | Mesocricetus auratus |
155000 | - |
polymerase IIb, gel filtration | Mesocricetus auratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | - |
CHO fibroblasts | - |
Purification (Comment) | Organism |
---|---|
polymerase IIa and IIb | Mesocricetus auratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | highly specific for ATP | Mesocricetus auratus | diphosphate + RNA(A)n | polymerase IIa: chain length of the product synthesized is independent of the primer concentration, polymerase IIb: the length of the product decreases when RNA concentration increases | ? | |
ATP + RNA | primer required | Mesocricetus auratus | diphosphate + RNA(A)n | polymerase IIa: chain length of the product synthesized is independent of the primer concentration, polymerase IIb: the length of the product decreases when RNA concentration increases | ? | |
ATP + RNA | polymerase IIa and IIb utilize a variety of natural and synthetic RNAs as well as DNA as primer | Mesocricetus auratus | diphosphate + RNA(A)n | polymerase IIa: chain length of the product synthesized is independent of the primer concentration, polymerase IIb: the length of the product decreases when RNA concentration increases | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 85000 + 1 * 60000, SDS-PAGE | Mesocricetus auratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
polymerase IIa and IIb | Mesocricetus auratus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6: 47% (polymerase IIa) and 5% (polymerase IIb) of maximum activity, pH 9: 55% of maximum activity | Mesocricetus auratus |