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Literature summary for 2.7.7.2 extracted from
- Probable reaction mechanisms of flavokinase and FAD synthetase from rat liver (1990), Arch. Biochem. Biophys., 278, 125-130.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | product inhibition | Rattus norvegicus |  |
| FAD | strong product inhibitor, 50% inhibition at 0.006 mM, competitive inhibition against ATP, mixed inhibition against FMN | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0091 | - |
FMN | pH 8.0, 37°C | Rattus norvegicus | |
| 0.071 | - |
ATP | pH 8.0, 37°C, MgATP | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | Rattus norvegicus | biosynthesis of FAD is most likely regulated by this coenzyme as a product at the stage of FAD synthetase reaction | diphosphate + FAD | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
rat | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatocyte | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | - |
Rattus norvegicus | diphosphate + FAD | - |
ir | |
| ATP + FMN | biosynthesis of FAD is most likely regulated by this coenzyme as a product at the stage of FAD synthetase reaction | Rattus norvegicus | diphosphate + FAD | - |
ir | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ki value for diphosphate against ATP not determined because Lineweaver-Burk plots of inhibition by diphosphate with varying concentrations of ATP are nonlinear | Rattus norvegicus | |
| 0.00075 | - |
FAD | pH 8.0, 37°C, against FMN | Rattus norvegicus | |
| 0.0013 | - |
FAD | pH 8.0, 37°C, against MgATP 2- | Rattus norvegicus | |
| 0.066 | - |
diphosphate | pH 8.0, 37°C, against FMN | Rattus norvegicus | |
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