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Literature summary for 2.7.7.2 extracted from
- Over-expression in Escherichia coli, purification and characterization of isoform 2 of human FAD synthetase (2007), Protein Expr. Purif., 52, 175-181.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain Rosetta(DE3) | Homo sapiens |
| expression in Escherichia coli | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00036 | - |
FMN | at 37°C | Homo sapiens |  |
| 0.0004 | - |
FMN | 37°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strict requirement for MgCl2 | Homo sapiens | |
| MgCl2 | strictly required | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE, T7-tagged enzyme | Homo sapiens |
| 55840 | - |
calculated from amino acid sequence, T7-tagged enzyme | Homo sapiens |
| 55844 | - |
x * 55000, SDS-PAGE, x * 55844, calculated | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q8NFF5 | - |
- |
| Homo sapiens | Q8NFF5 | isoform Fads2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| recombinant protein | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.03 | - |
at 37°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | - |
Homo sapiens | diphosphate + FAD | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE, x * 55844, calculated | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAD synthetase isoform 2 | - |
Homo sapiens |
| FADS2 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0036 | - |
FMN | 37°C | Homo sapiens | |
| 0.0036 | - |
FMN | at 37°C | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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