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Literature summary for 2.7.7.23 extracted from
- Identification and characterization of a strict and a promiscuous N-acetylglucosamine-1-P uridylyltransferase in Arabidopsis (2010), Biochem. J., 430, 275-284.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arabidopsis thaliana |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| generation of a three-dimensional model, using human GlcNAc1p nucleotidyltransferase complexed with UDP-GlcNAc, PDB code 1JV1 | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | 0.5 mM, 56% reduction in activity | Arabidopsis thaliana |  |
| EDTA | 5 mM, 11% residual activity | Arabidopsis thaliana | |
| hygromycin | 0.5 mg/ml, 36% reduction in activity | Arabidopsis thaliana | |
| streptomycin | 0.5 mg/ml, 74% reduction in activity | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
UDP-N-acetyl-D-glucosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
| 0.18 | - |
N-acetyl-D-glucosamine 1-phosphate | pH 7.6, 37°C | Arabidopsis thaliana | |
| 0.203 | - |
UTP | pH 7.6, 37°C | Arabidopsis thaliana | |
| 0.219 | - |
UDP-N-acetyl-D-glucosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
| 0.296 | - |
UTP | pH 7.6, 37°C | Arabidopsis thaliana | |
| 0.337 | - |
N-acetyl-D-glucosamine 1-phosphate | pH 7.6, 37°C | Arabidopsis thaliana | |
| 0.808 | - |
UDP-N-acetyl-D-galactosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
| 2.768 | - |
UDP-N-acetyl-D-galactosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | or Mn2+, required | Arabidopsis thaliana | |
| Mn2+ | or Mg2+, required | Arabidopsis thaliana | |
| Mn2+ | or Mg2+, required. At 5 mM, 126% of the activity with Mg2+ | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58300 | - |
1 * 58300, SDS-PAGE | Arabidopsis thaliana |
| 60000 | - |
gel filtration | Arabidopsis thaliana |
| 60000 | - |
x * 60000, SDS-PAGE | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | O64765 | isoform GLCNAC1PUT2 | - |
| Arabidopsis thaliana | Q940S3 | isoform GLCNAC1PUT1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme additionally shows the activity of UTP-glucose-1-phosphate uridylyltransferase, EC 2.7.7.9 | Arabidopsis thaliana | ? | - |
? | |
| additional information | no substrates: CTP, GTP, ITP or ATP, glucose 1-phosphate, glucosamine 1-phosphate, xylose 1-phosphate, galactose 1-phosphate, galactosamin 1-phosphate, fucose 1-phosphate, mannose 1-phosphate, galactosamine 1-phosphate, fructose 1-phosphate, or glucose 6-phosphate | Arabidopsis thaliana | ? | - |
? | |
| N-acetyl-D-glucosamine 1-phosphate + UTP | - |
Arabidopsis thaliana | UDP-N-acetyl-D-glucosamine + diphosphate | - |
r | |
| UDP-N-acetyl-D-galactosamine + diphosphate | - |
Arabidopsis thaliana | N-acetyl-D-galactosamine 1-phosphate + UTP | - |
r | |
| UDP-N-acetyl-D-glucosamine + diphosphate | - |
Arabidopsis thaliana | N-acetyl-D-glucosamine 1-phosphate + UTP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Arabidopsis thaliana |
| monomer | 1 * 58300, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GLCNAC1PUT1 | - |
Arabidopsis thaliana |
| GLCNAC1PUT2 | - |
Arabidopsis thaliana |
| UDP-N-acetylglucosamine diphosphorylase 1 | - |
Arabidopsis thaliana |
| UDP-N-acetylglucosamine diphosphorylase 2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | - |
Arabidopsis thaliana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
65 | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8 | - |
Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.3 | 9 | - |
Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15 | - |
UDP-N-acetyl-D-galactosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
| 18 | - |
UDP-N-acetyl-D-galactosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
| 110 | - |
N-acetyl-D-glucosamine 1-phosphate | pH 7.6, 37°C | Arabidopsis thaliana | |
| 120 | - |
UTP | pH 7.6, 37°C | Arabidopsis thaliana | |
| 140 | - |
UTP | pH 7.6, 37°C | Arabidopsis thaliana | |
| 160 | - |
N-acetyl-D-glucosamine 1-phosphate | pH 7.6, 37°C | Arabidopsis thaliana | |
| 220 | - |
UDP-N-acetyl-D-glucosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
| 460 | - |
UDP-N-acetyl-D-glucosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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