Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, wild-type and truncated enzyme form lacking the 170-residues C-terminal domain | Sulfurisphaera tokodaii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a truncated enzyme form lacking the 170-residues C-terminal domain has decreased thermostability | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
dTTP | pH 7.5, 80°C | Sulfurisphaera tokodaii | |
0.05 | - |
dTDP-alpha-D-glucose | pH 7.5, 80°C | Sulfurisphaera tokodaii | |
0.39 | - |
diphosphate | pH 7.5, 80°C | Sulfurisphaera tokodaii | |
1.12 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 80°C | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+ | Sulfurisphaera tokodaii | |
Mg2+ | absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+. Optimal Mg2+ concentration is 6 mM | Sulfurisphaera tokodaii | |
Mn2+ | absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+ | Sulfurisphaera tokodaii | |
Zn2+ | absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+ | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q975F9 | - |
- |
Sulfurisphaera tokodaii 7 | Q975F9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.35 | - |
pH 7.5, 80°C, substrates: dTTP + alpha-D-glucose 1-phosphate | Sulfurisphaera tokodaii |
9.4 | - |
pH 7.5, 80°C, substrates: diphosphate + dTDP-alpha-D-glucose | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + alpha-D-glucose 1-phosphate | maximal velocity of the reverse reaction is 11.4fold of the maximal velocity of the forward reaction. The multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.9, UTP-glucose-1-phosphate uridylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + N-acetylglucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dGTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate guanylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate | Sulfurisphaera tokodaii | diphosphate + dTDP-alpha-D-glucose | - |
r | |
dTTP + alpha-D-glucose 1-phosphate | maximal velocity of the reverse reaction is 11.4fold of the maximal velocity of the forward reaction. The multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.9, UTP-glucose-1-phosphate uridylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + N-acetylglucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dGTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate guanylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate | Sulfurisphaera tokodaii 7 | diphosphate + dTDP-alpha-D-glucose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ST0452 | locus name | Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Sulfurisphaera tokodaii |
95 | - |
- |
Sulfurisphaera tokodaii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | 70°C: about 40% of maximal activity, 100°C: about 80% of maximal activity | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 180 min | Sulfurisphaera tokodaii |
95 | - |
half-life: 60 min | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sulfurisphaera tokodaii |
8.5 | - |
- |
Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity | Sulfurisphaera tokodaii |