Literature summary for 2.7.7.27 extracted from

Sowokinos, J.R.; Preiss, J.
Pyrophphosphorylases in Solanum tuberosum, III. Purification, physical, and catalytic properties of ADPglucose pyrophophorylase in potatoes (1982), Plant Physiol., 69, 1459-1466.

Search for more literature for 2.7.7.27

Activating Compound

Activating Compound	Comment	Organism	Structure
3-phosphoglycerate	allosteric activation	Solanum tuberosum
3-phosphoglycerate	activation	Solanum tuberosum
alpha-glycerol phosphate	activation, pyrophosphorolysis, not	Solanum tuberosum
D-fructose 1,6-bisphosphate	slight activation	Solanum tuberosum
D-fructose 1,6-bisphosphate	activation of ADP-glucose synthesis	Solanum tuberosum
D-ribose 5-phosphate	activation	Solanum tuberosum
D-ribose 5-phosphate	activation of ADP-glucose synthesis	Solanum tuberosum
DTT	activation	Solanum tuberosum
additional information	not: L-lactate, citrate	Solanum tuberosum
additional information	not: oxaloacetate, L-malate	Solanum tuberosum
additional information	not: 2-oxoglutarate	Solanum tuberosum
phosphoenolpyruvate	activation	Solanum tuberosum
phosphoenolpyruvate	activation of pyrophosphorolysis	Solanum tuberosum
phosphoenolpyruvate	ADPglucose synthesis	Solanum tuberosum
pyruvate	not	Solanum tuberosum

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	less effective than phosphate	Solanum tuberosum
AMP	less effective than phosphate	Solanum tuberosum
cyclic-3',5'-AMP	less effective than phosphate	Solanum tuberosum
NADP+	less effective than phosphate	Solanum tuberosum
phosphate	kinetics; strong	Solanum tuberosum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.14	-	alpha-D-glucose 1-phosphate	pH 8.0, 37°C	Solanum tuberosum
0.19	-	ATP	pH 8.0, 37°C	Solanum tuberosum
0.24	-	ADP-glucose	pH 8.0, 37°C	Solanum tuberosum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	requirement	Solanum tuberosum
Mg2+	ADPglucose synthesis	Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	4 * 50000, SDS-PAGE	Solanum tuberosum
200000	-	sucrose density gradient centrifugation	Solanum tuberosum

Organism

Organism	UniProt	Comment	Textmining
Solanum tuberosum	-	potato, var. Norchip	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Solanum tuberosum

Source Tissue

Source Tissue	Comment	Organism	Textmining
tuber	growing	Solanum tuberosum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.8	-	-	Solanum tuberosum

Storage Stability

Storage Stability	Organism
4°C, in 50 mM HEPES buffer, pH 7.5, 20% sucrose, 1 mM EDTA, 2 mM GSH, at least 6 weeks	Solanum tuberosum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + alpha-D-glucose 1-phosphate	-	Solanum tuberosum	diphosphate + ADP-glucose	-	r

Subunits

Subunits	Comment	Organism
homotetramer	4 * 50000, SDS-PAGE	Solanum tuberosum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	HEPES, bicine and glycylglycine buffer	Solanum tuberosum
8	-	ADP-glucose synthesis	Solanum tuberosum
8.5	-	Tris buffer	Solanum tuberosum
8.5	-	ADP-glucose synthesis	Solanum tuberosum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)