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Literature summary for 2.7.7.27 extracted from
- Biosynthesis of bacterial glycogen. Characterization of the subunit structure of Escherichia coli B glucose-1-phosphate adenylyltransferase (EC 2.7.7.27) (1976), J. Biol. Chem., 251, 7880-7885.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-bisphosphate | allosteric activator | Escherichia coli |  |
General Stability
| General Stability | Organism |
|---|---|
| irreversibly insoluble when dialyzed against deionized water | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Escherichia coli | |
| phosphate | allosteric inhibitor | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 37°C, strain AC70R1, kinetic study | Escherichia coli | |
| 0.25 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 37°C, strain B, kinetic study | Escherichia coli | |
| 1.2 | - |
ATP | pH 7.0, 37°C, strain AC70R1, kinetic study | Escherichia coli | |
| 1.3 | - |
ATP | pH 7.0, 37°C, strain B, kinetic study | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition and comparison | Escherichia coli |
| 50000 | - |
4 * 50000, SDS-PAGE | Escherichia coli |
| 185000 | - |
mutant AC70R1, sedimentation velocity measurement | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli | - |
mutant strain AC70R1 | - |
| Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutant AC70R1, affinity chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 90 | 110 | - |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 50 mM Tris-HCl, pH 7.2, 2.5 mM dithioerythritol, at least 1 year | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + ADP-glucose | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli B / ATCC 11303 | diphosphate + ADP-glucose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 50000, SDS-PAGE | Escherichia coli |
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