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Literature summary for 2.7.7.27 extracted from

  • Ballicora, M.A.; Sesma, J.I.; Iglesias, A.A.; Preiss, J.
    Characterization of Chimeric ADPglucose Pyrophosphorylases of Escherichia coli and Agrobacterium tumefaciens. Importance of the C-Terminus on the Selectivity for Allosteric Regulators (2002), Biochemistry, 41, 9431-9437.
    View publication on PubMed
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-bisphosphate mainly Escherichia coli and chimeric enzyme AE containing the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, not chimeric enzyme EA containing the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme, C-terminus of Escherichia coli is relavant for the selectivity Escherichia coli
D-fructose 1,6-bisphosphate mainly Escherichia coli and chimeric enzyme AE containing the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, not chimeric enzyme EA containing the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme, C-terminus of Escherichia coli is relavant for the selectivity Agrobacterium tumefaciens
D-fructose 6-phosphate mainly Agrobacterium tumefaciens and chimeric enzyme AE containing the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, not chimeric enzyme EA containing the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme Escherichia coli
D-fructose 6-phosphate mainly Agrobacterium tumefaciens and chimeric enzyme AE containing the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, not chimeric enzyme EA containing the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme Agrobacterium tumefaciens
pyruvate activation of chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme with higher affinity than activation of chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme Escherichia coli
pyruvate activation of chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme with higher affinity than activation of chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme Agrobacterium tumefaciens

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and two chimeric enzymes Escherichia coli
expression of wild-type and two chimeric enzymes Agrobacterium tumefaciens

Protein Variants

Protein Variants Comment Organism
additional information construction of two chimeric enzymes, AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme and EA is the inverse construction, chimeric enzyme AE is activated by D-fructose 1,6-bisphosphate, D-fructose 6-phosphate and pyruvate, chimeric enzyme AE is only activated by pyruvate Escherichia coli
additional information construction of two chimeric enzymes, AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme and EA is the inverse construction, chimeric enzyme AE is activated by D-fructose 1,6-bisphosphate, D-fructose 6-phosphate and pyruvate, chimeric enzyme AE is only activated by pyruvate Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
AMP comparison of inhibition of wild-type and chimeric enzymes Agrobacterium tumefaciens
AMP comparison of inhibition of wild-type and chimeric enzymes Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.086
-
 ATP pH 8.0, 37°C, wild-type enzyme Agrobacterium tumefaciens
0.155
-
 ATP pH 8.0, 37°C, chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme Escherichia coli
0.155
-
 ATP pH 8.0, 37°C, chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme Agrobacterium tumefaciens
0.162
-
 ATP pH 8.0, 37°C, chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme Escherichia coli
0.162
-
 ATP pH 8.0, 37°C, chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme Agrobacterium tumefaciens
0.3
-
 ATP pH 8.0, 37°C, wild-type enzyme Escherichia coli
0.39
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme Escherichia coli
0.39
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme Agrobacterium tumefaciens
0.45
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, wild-type enzyme Escherichia coli
0.6
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, wild-type enzyme Agrobacterium tumefaciens
0.92
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme Escherichia coli
0.92
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme Agrobacterium tumefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Escherichia coli
Mg2+ requirement Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Escherichia coli
additional information
-
-
 Agrobacterium tumefaciens
50000
-
 4 * 50000, SDS-PAGE Escherichia coli
50000
-
 4 * 50000, SDS-PAGE Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate Escherichia coli key regulatory enzyme of starch biosynthesis diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate Agrobacterium tumefaciens key regulatory enzyme of starch biosynthesis diphosphate + ADP-glucose
-
 r

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and two chimeric enzymes Escherichia coli
wild-type and two chimeric enzymes Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27
-
 chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme Escherichia coli
27
-
 chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme Agrobacterium tumefaciens
36
-
 chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme Escherichia coli
36
-
 chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme Agrobacterium tumefaciens
39
-
 large and small subunits Escherichia coli
39
-
 large and small subunits Agrobacterium tumefaciens
129
-
 wild-type enzyme Agrobacterium tumefaciens
148
-
 wild-type enzyme Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, in 50 mM HEPES buffer, pH 8.0, 10% sucrose, 5mM MgCl2, 0.1 mM EDTA, stable for at least 3 months Escherichia coli
-20°C, in 50 mM HEPES buffer, pH 8.0, 10% sucrose, 5mM MgCl2, 0.1 mM EDTA, stable for at least 3 months Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Escherichia coli diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate
-
 Agrobacterium tumefaciens diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate key regulatory enzyme of starch biosynthesis Escherichia coli diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate key regulatory enzyme of starch biosynthesis Agrobacterium tumefaciens diphosphate + ADP-glucose
-
 r

Subunits

Subunits Comment Organism
homotetramer 4 * 50000, SDS-PAGE Escherichia coli
homotetramer 4 * 50000, SDS-PAGE Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 87% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 75% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 60% remaining activity of wild-type enzymes Escherichia coli
50
-
 87% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 75% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 60% remaining activity of wild-type enzymes Agrobacterium tumefaciens
55
-
 65% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 70% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 90% remaining activity of wild-type enzymes Escherichia coli
55
-
 65% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 70% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 90% remaining activity of wild-type enzymes Agrobacterium tumefaciens
60
-
 37% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 45% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 70% remaining activity of wild-type enzymes Escherichia coli
60
-
 37% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 45% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 70% remaining activity of wild-type enzymes Agrobacterium tumefaciens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
 AMP pH 8.0, 37°C, wild-type enzyme in presence of 0.1 mM fructose-1,6-bisphosphate Escherichia coli
3
-
 AMP pH 8.0, 37°C, wild-type enzyme in presence of 0.3 mM fructose-1,6-bisphosphate Agrobacterium tumefaciens
9
-
 AMP pH 8.0, 37°C, the chimeric enzymes, AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme is slightly activated by AMP in the range of 0.1-2 mM Escherichia coli
9
-
 AMP pH 8.0, 37°C, the chimeric enzymes, AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme is slightly activated by AMP in the range of 0.1-2 mM Agrobacterium tumefaciens