Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | - |
Zea mays | |
3-phosphoglycerate | - |
Solanum tuberosum | |
D-fructose 1,6-bisphosphate | - |
Zea mays | |
D-fructose 1,6-bisphosphate | - |
Solanum tuberosum | |
D-fructose 6-phosphate | - |
Zea mays | |
D-fructose 6-phosphate | - |
Solanum tuberosum |
Cloned (Comment) | Organism |
---|---|
wild-type gene pML10 is mutated by hydroxylamine, the mutated SS gene TG-15 is expressed in Escherichia coli AC70R1-504 | Solanum tuberosum |
Protein Variants | Comment | Organism |
---|---|---|
L48F/V59I | TG-15, significant alteration in effector sensitivity of this homotetrameric enzyme in comparison to wild-type heterotetrameric enzyme | Solanum tuberosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Solanum tuberosum | |
phosphate | - |
Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Zea mays | |
additional information | - |
additional information | - |
Solanum tuberosum | |
0.25 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 37°C, mutant TG-15, kinetic study | Solanum tuberosum | |
1.2 | - |
ATP | pH 7.5, 37°C, mutant TG-15, kinetic study | Solanum tuberosum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Solanum tuberosum | |
Mg2+ | requirement | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition and comparison | Zea mays |
additional information | - |
amino acid composition and comparison | Synechocystis sp. |
additional information | - |
amino acid composition and comparison | Solanum tuberosum |
additional information | - |
amino acid composition and comparison | Anabaena sp. |
additional information | - |
amino acid composition and comparison | Escherichia coli |
50000 | - |
4 * 50000, the small subunit enzyme TG-15, SDS-PAGE | Solanum tuberosum |
200000 | - |
- |
Zea mays |
200000 | - |
gel filtration of homotetramer small subunit enzyme TG-15 | Solanum tuberosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena sp. | P30521 | - |
- |
Escherichia coli | P0A6V1 | glgC | - |
Solanum tuberosum | P23509 | SS | - |
Solanum tuberosum SS | P23509 | SS | - |
Synechocystis sp. | P52415 | - |
- |
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, homotetrameric small subunit enzyme TG-15, heat shock step at 55°C | Solanum tuberosum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-alpha-D-glucose | the small and large subunits of the enzyme define the catalytic and regulatory subunits | Solanum tuberosum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Solanum tuberosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Synechocystis sp. | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Anabaena sp. | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum SS | diphosphate + ADP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Zea mays |
homotetramer | 4 * 50000, the small subunit enzyme TG-15, SDS-PAGE | Solanum tuberosum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
purification of small subunit enzyme TG-15 involves a 7 min/55°C heat shock step | Solanum tuberosum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of Ki of phosphate | Solanum tuberosum |