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Literature summary for 2.7.7.27 extracted from
- Relative turnover numbers of maize endosperm and potato tuber ADP-glucose pyrophosphorylases in the absence and presence of 3-phosphoglyceric acid (2003), Planta, 217, 449-456.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | - |
Oryza sativa |  |
| 3-phosphoglycerate | the activities of recombinant maize and recombinant potato enzyme are comparable in the presence of 10 mM 3-phosphoglycerate, however in absence of 3-phosphoglycerate and phosphate the maize enzyme exhibits approximately 20fold more activity than does potato enzyme, in presence of both 3-phosphoglycerate and phosphate the maize enzyme is 47fold active than is the potato enzyme | Zea mays | |
| 3-phosphoglycerate | the activities of recombinant maize and recombinant potato enzyme are comparable in the presence of 10 mM 3-phosphoglycerate, however in absence of 3-phosphoglycerate and phosphate the maize enzyme exhibits approximately 20fold more activity than does potato enzyme, in presence of both 3-phosphoglycerate and phosphate the maize enzyme is 47fold active than is the potato enzyme | Solanum tuberosum | |
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | genetic manipulation of enzyme to enhance rice seed yield and plant biomass | Oryza sativa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli AC70R1-504 | Zea mays |
| expression in Escherichia coli AC70R1-504 | Solanum tuberosum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L38E | UpReg-1, this mutation greatly increases activation by 3-phosphoglycerate | Zea mays |
| L93Thr | Sh2-UR1, this mutation does not alter 3-phosphoglycerate activation and phosphate inhibition | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Oryza sativa | |
| phosphate | - |
Solanum tuberosum | |
| phosphate | - |
Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
- |
Zea mays |
| 200000 | - |
gel filtration | Solanum tuberosum |
| 220000 | - |
gel filtration | Zea mays |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | Zea mays | key regulatory enzyme of starch biosynthesis | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | Solanum tuberosum | key regulatory enzyme of starch biosynthesis | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | Oryza sativa | key regulatory enzyme of starch biosynthesis | diphosphate + ADP-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
| Solanum tuberosum | - |
- |
- |
| Zea mays | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | substitution of two lysine residues into maize enzyme to produce a variant containing the three lysines found in the large subunit of potato enzyme has no effect on 3-phosphoglycerate activation of the maize enzyme | Zea mays |
| side-chain modification | substitution of two lysine residues into maize enzyme to produce a variant containing the three lysines found in the large subunit of potato enzyme has no effect on 3-phosphoglycerate activation of the maize enzyme | Solanum tuberosum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Zea mays |
| recombinant enzyme | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Zea mays | - |
| endosperm | - |
Solanum tuberosum | - |
| endosperm | - |
Oryza sativa | - |
| tuber | - |
Solanum tuberosum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Oryza sativa | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | key regulatory enzyme of starch biosynthesis | Zea mays | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | key regulatory enzyme of starch biosynthesis | Solanum tuberosum | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | key regulatory enzyme of starch biosynthesis | Oryza sativa | diphosphate + ADP-glucose | - |
? | |
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