Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Escherichia coli | |
AMP | activation at pH 7 | Cereibacter sphaeroides | |
D-fructose 1,6-bisphosphate | - |
Escherichia coli | |
D-fructose 1,6-bisphosphate | - |
Cereibacter sphaeroides | |
D-fructose 1,6-bisphosphate | - |
Anabaena sp. | |
D-fructose 6-phosphate | - |
Escherichia coli | |
D-fructose 6-phosphate | - |
Cereibacter sphaeroides | |
D-fructose 6-phosphate | - |
Anabaena sp. | |
pyruvate | - |
Cereibacter sphaeroides |
Cloned (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Cereibacter sphaeroides | |
ADP | - |
Escherichia coli | |
AMP | AMP activation at pH 7, AMP acting as a competitive ligand for an allosteric site at pH 8 where both activators and inhibitors bind and mediate their effect | Cereibacter sphaeroides | |
AMP | - |
Escherichia coli | |
phosphate | - |
Anabaena sp. | |
phosphate | the presence of 2.5 mM D-fructose 6-phosphate completely prevents phosphate inhibition, in the presence of 1 mM phosphate, inhibition by phosphoenolpyruvate drops to 50% | Cereibacter sphaeroides | |
phosphate | - |
Escherichia coli | |
phosphoenolpyruvate | in the presence of 1 mM phosphate, inhibition by the 1 mM phosphoenolpyruvate drops to 50%, inhibition is reduced to 20% in the presence of 0.15 mM fructose 6-phosphate | Cereibacter sphaeroides | |
phosphoenolpyruvate | - |
Escherichia coli | |
pyridoxal 5'-phosphate | - |
Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
alpha-D-glucose 1-phosphate | pH 8, 37°C, in absence of fructose 6-phosphate | Cereibacter sphaeroides | |
0.056 | - |
alpha-D-glucose 1-phosphate | pH 8, 37°C, in presence of fructose 6-phosphate | Cereibacter sphaeroides | |
0.079 | - |
ATP | pH 8, 37°C, in presence of fructose 6-phosphate | Cereibacter sphaeroides | |
0.22 | - |
ATP | pH 8, 37°C, enzyme modified for 30 min with 5 mM 2,3-butanedione | Cereibacter sphaeroides | |
0.4 | - |
ATP | pH 8, 37°C, in absence of fructose 6-phosphate | Cereibacter sphaeroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Escherichia coli | |
Mg2+ | requirement | Cereibacter sphaeroides | |
Mg2+ | requirement | Anabaena sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
N-terminal sequence and comparison | Escherichia coli |
additional information | - |
N-terminal sequence and comparison | Cereibacter sphaeroides |
additional information | - |
amino acid composition and comparison | Escherichia coli |
additional information | - |
amino acid composition and comparison | Cereibacter sphaeroides |
additional information | - |
amino acid composition and comparison | Anabaena sp. |
51000 | - |
2 * 51000 | Cereibacter sphaeroides |
110000 | - |
gel filtration | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena sp. | - |
- |
- |
Cereibacter sphaeroides | - |
- |
- |
Cereibacter sphaeroides | Q9RNH7 | 2.4.1 | - |
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | - |
Escherichia coli |
side-chain modification | chemical modification of the purified enzyme with the arginyl reagents 2,3 butanedione and phenylglyoxal results in desensitation of the enzyme to both activators and inhibitors, affinity to ATP of enzyme modified 30 min with 5 mM 2,3-butanedione increases 3fold in comparison to the unmodified enzyme | Cereibacter sphaeroides |
Purification (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
172 | - |
- |
Cereibacter sphaeroides |
Storage Stability | Organism |
---|---|
-80°C, stable for more than 3 months | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Cereibacter sphaeroides | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Anabaena sp. | diphosphate + ADP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 51000 | Cereibacter sphaeroides |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
phosphoenolpyruvate | pH 8.0, 37°C | Cereibacter sphaeroides | |
0.37 | - |
phosphate | pH 8.0, 37°C | Cereibacter sphaeroides | |
0.51 | - |
ADP | pH 8.0, 37°C | Cereibacter sphaeroides |