Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-diphosphate | 50% of maximal activation at 0.059 for wild-type | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
homology model of enzyme strucutre complexed with ADP-glucose | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D239A | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
D239E | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
D239N | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
D276A | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
D276E | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
D276N | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
E194A | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
E194D | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
E194Q | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
F240A | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
F240M | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
K195Q | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
S212A | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
S212T | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
S212V | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
S212Y | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
W274A | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
W274F | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
W274L | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
Y216F | residue in close proximity to the glucose moiety of ADP-glucose substrate. Significant decrease in affinity for glucose 1-phosphate, kinetic analysis | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
alpha-D-glucose 1-phosphate | wild-type, pH 8.0, 37°C | Escherichia coli | |
0.05 | - |
alpha-D-glucose 1-phosphate | mutant W274F, pH 8.0, 37°C | Escherichia coli | |
0.09 | - |
alpha-D-glucose 1-phosphate | mutant S212Y, pH 8.0, 37°C | Escherichia coli | |
0.122 | - |
alpha-D-glucose 1-phosphate | mutant F240M, pH 8.0, 37°C | Escherichia coli | |
0.16 | - |
ATP | mutant D239A, pH 8.0, 37°C | Escherichia coli | |
0.169 | - |
alpha-D-glucose 1-phosphate | mutant D239E, pH 8.0, 37°C | Escherichia coli | |
0.17 | - |
ATP | mutant E194Q, pH 8.0, 37°C | Escherichia coli | |
0.19 | - |
ATP | mutant K195Q, pH 8.0, 37°C | Escherichia coli | |
0.204 | - |
alpha-D-glucose 1-phosphate | mutant F240A, pH 8.0, 37°C | Escherichia coli | |
0.24 | - |
alpha-D-glucose 1-phosphate | mutant S212A, pH 8.0, 37°C | Escherichia coli | |
0.264 | - |
alpha-D-glucose 1-phosphate | mutant D239N, pH 8.0, 37°C | Escherichia coli | |
0.28 | - |
ATP | mutant W274F, pH 8.0, 37°C | Escherichia coli | |
0.35 | - |
ATP | mutant Y216F, pH 8.0, 37°C | Escherichia coli | |
0.367 | - |
alpha-D-glucose 1-phosphate | mutant W274A, pH 8.0, 37°C | Escherichia coli | |
0.38 | - |
ATP | mutant S212V, pH 8.0, 37°C | Escherichia coli | |
0.41 | - |
alpha-D-glucose 1-phosphate | mutant D276E, pH 8.0, 37°C | Escherichia coli | |
0.41 | - |
ATP | mutant S212T, pH 8.0, 37°C | Escherichia coli | |
0.43 | - |
ATP | mutant S212Y, pH 8.0, 37°C | Escherichia coli | |
0.48 | - |
ATP | mutant W274L, pH 8.0, 37°C | Escherichia coli | |
0.49 | - |
ATP | mutant E194D, pH 8.0, 37°C | Escherichia coli | |
0.524 | - |
alpha-D-glucose 1-phosphate | mutant D239A, pH 8.0, 37°C | Escherichia coli | |
0.525 | - |
alpha-D-glucose 1-phosphate | mutant W274L, pH 8.0, 37°C | Escherichia coli | |
0.56 | - |
ATP | mutant D239N, pH 8.0, 37°C | Escherichia coli | |
0.59 | - |
ATP | wild-type, pH 8.0, 37°C | Escherichia coli | |
0.68 | - |
ATP | mutant S212A, pH 8.0, 37°C | Escherichia coli | |
0.785 | - |
alpha-D-glucose 1-phosphate | mutant Y216F, pH 8.0, 37°C | Escherichia coli | |
0.96 | - |
ATP | mutant D239E, pH 8.0, 37°C | Escherichia coli | |
1.04 | - |
ATP | mutant W274A, pH 8.0, 37°C | Escherichia coli | |
1.14 | - |
ATP | mutant F240A, pH 8.0, 37°C | Escherichia coli | |
1.2 | - |
ATP | mutant E194A, pH 8.0, 37°C | Escherichia coli | |
1.44 | - |
alpha-D-glucose 1-phosphate | mutant E194Q, pH 8.0, 37°C | Escherichia coli | |
1.45 | - |
alpha-D-glucose 1-phosphate | mutant D276N, pH 8.0, 37°C | Escherichia coli | |
1.7 | - |
alpha-D-glucose 1-phosphate | mutant D276A, pH 8.0, 37°C | Escherichia coli | |
1.98 | - |
ATP | mutant F240M, pH 8.0, 37°C | Escherichia coli | |
2.03 | - |
ATP | mutant D276A, pH 8.0, 37°C | Escherichia coli | |
2.3 | - |
ATP | mutant D276N, pH 8.0, 37°C | Escherichia coli | |
2.81 | - |
alpha-D-glucose 1-phosphate | mutant E194A, pH 8.0, 37°C | Escherichia coli | |
4.66 | - |
alpha-D-glucose 1-phosphate | mutant S212T, pH 8.0, 37°C | Escherichia coli | |
4.77 | - |
ATP | mutant D276E, pH 8.0, 37°C | Escherichia coli | |
6.42 | - |
alpha-D-glucose 1-phosphate | mutant S212V, pH 8.0, 37°C | Escherichia coli | |
6.59 | - |
alpha-D-glucose 1-phosphate | mutant E194D, pH 8.0, 37°C | Escherichia coli | |
16.7 | - |
alpha-D-glucose 1-phosphate | mutant K195Q, pH 8.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Km value for wild-type 2.6 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6V1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + ADP-glucose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, 85% residual activity for wild-type | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
ATP | mutant D276A, pH 8.0, 37°C | Escherichia coli | |
0.37 | - |
ATP | mutant D276N, pH 8.0, 37°C | Escherichia coli | |
0.52 | 0.7 | ATP | mutant E194Q, pH 8.0, 37°C | Escherichia coli | |
1.6 | - |
ATP | mutant S212Y, pH 8.0, 37°C | Escherichia coli | |
15.4 | - |
ATP | mutant E194A, pH 8.0, 37°C | Escherichia coli | |
22.4 | - |
ATP | mutant S212V, pH 8.0, 37°C | Escherichia coli | |
27 | - |
ATP | mutant D276E, pH 8.0, 37°C | Escherichia coli | |
29 | - |
ATP | mutant Y216F, pH 8.0, 37°C | Escherichia coli | |
32.7 | - |
ATP | mutant D239A, pH 8.0, 37°C | Escherichia coli | |
80.7 | - |
ATP | mutant E194Q, pH 8.0, 37°C | Escherichia coli | |
92.7 | - |
ATP | mutant E194D, pH 8.0, 37°C | Escherichia coli | |
104 | - |
ATP | mutant E194D, pH 8.0, 37°C | Escherichia coli | |
112.7 | - |
ATP | mutant D276E, pH 8.0, 37°C | Escherichia coli | |
169 | - |
ATP | mutant D239N, pH 8.0, 37°C | Escherichia coli | |
171.7 | - |
ATP | mutant F240A, pH 8.0, 37°C | Escherichia coli | |
179 | - |
ATP | mutant S212T, pH 8.0, 37°C | Escherichia coli | |
193.3 | - |
ATP | mutant K195Q, pH 8.0, 37°C | Escherichia coli | |
247.5 | - |
ATP | mutant W274L, pH 8.0, 37°C | Escherichia coli | |
266 | - |
ATP | mutant W274F, pH 8.0, 37°C | Escherichia coli | |
347.7 | - |
ATP | mutant D239E, pH 8.0, 37°C | Escherichia coli | |
370 | - |
ATP | wild-type, pH 8.0, 37°C | Escherichia coli | |
371.2 | - |
ATP | mutant S212A, pH 8.0, 37°C | Escherichia coli | |
384 | - |
ATP | mutant W274A, pH 8.0, 37°C | Escherichia coli | |
487 | - |
ATP | mutant F240M, pH 8.0, 37°C | Escherichia coli |