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Literature summary for 2.7.7.27 extracted from

  • Cupp-Vickery, J.R.; Igarashi, R.Y.; Perez, M.; Poland, M.; Meyer, C.R.
    Structural analysis of ADP-glucose pyrophosphorylase from the bacterium Agrobacterium tumefaciens (2008), Biochemistry, 47, 4439-4451.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
fructose 6-phosphate is an allosteric activator, binds to the interface between the N- and C-terminal domains, binding structure, modelling Agrobacterium tumefaciens

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of selenomethionyl-labeled wild-type and mutant enzymes in glgC-Escherichia coli cells, strain TGC3 Agrobacterium tumefaciens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant selenomethionyl-labeled enzyme anomalous selenomethionyl derivative, sitting drop vapor diffusion method, lithium sulfate as a precipitant, samples containing 5 mg/ml protein are mixed with an equal volume of 1.5 M lithium sulfate and 100 mM HEPES, pH 7.5, as the mother liquor, 1 week, mother liquor containing 20% v/v glycerol for cryoprotection, X-ray diffraction structure determination and analysis at 2.1 A resolution, modelling with ATP and alpha-D-glucose-1-phosphate bound to the active site, overview Agrobacterium tumefaciens

Protein Variants

Protein Variants Comment Organism
H379K site-directed mutagenesis, kinetics compared to the wild-type enzyme Agrobacterium tumefaciens
H379R site-directed mutagenesis, kinetics compared to the wild-type enzyme Agrobacterium tumefaciens
additional information site-directed mutagenesis of conserved glycines in this region, G20, G21, and G23, results in substantial loss of activity Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
sulfate is a competitive inhibitor for the allosteric activator fructose 6-phosphate, binds to the interface between the N- and C-terminal domains, binding structure, modelling Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information wild-type and ADPGlc PPase H379R and ADPGlc PPase H379K mutant kinetics, overview Agrobacterium tumefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate Agrobacterium tumefaciens key step in glucan synthesis, the ADPGlc PPases are highly regulated by allosteric activators and inhibitors in accord with the carbon metabolism pathways of the organism ADP-D-glucose + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens P39669
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant selenomethionyl-labeled wild-type and mutant enzymes from glgC-Escherichia coli cells, strain TGC3 Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
Agrobacterium tumefaciens ADP-D-glucose + diphosphate
-
r
ATP + alpha-D-glucose 1-phosphate key step in glucan synthesis, the ADPGlc PPases are highly regulated by allosteric activators and inhibitors in accord with the carbon metabolism pathways of the organism Agrobacterium tumefaciens ADP-D-glucose + diphosphate
-
r

Subunits

Subunits Comment Organism
More two domains: an N-terminal alpha/alpha sandwich and a C-terminal parallel beta-helix, ADPGlc PPase/fructose 6-phosphate structural model, overview Agrobacterium tumefaciens

Synonyms

Synonyms Comment Organism
ADP-glucose pyrophosphorylase
-
Agrobacterium tumefaciens
ADPGlc PPase
-
Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Agrobacterium tumefaciens