Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | 3-PGA | Solanum tuberosum | |
3-phosphoglycerate | 3-PGA | Zea mays |
Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli AC70R1-504 cells | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
A508S | large subunit mutation | Zea mays |
C114A | large subunit mutation | Zea mays |
C382F | large subunit mutation | Zea mays |
C424V | large subunit mutation | Zea mays |
D368S | large subunit mutation | Zea mays |
E438Q | large subunit mutation | Zea mays |
H149S | large subunit mutation | Zea mays |
M172T | large subunit mutation | Zea mays |
P372A | large subunit mutation | Zea mays |
Q213H | large subunit mutation | Zea mays |
S163F | large subunit mutation | Zea mays |
T361C | large subunit mutation | Zea mays |
V227R | large subunit mutation | Zea mays |
V502T | large subunit mutation | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
inorganic phosphate | - |
Solanum tuberosum | |
phosphate | - |
Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
alpha-D-glucose 1-phosphate | mutant BT2/E438Q | Zea mays | |
0.05 | - |
alpha-D-glucose 1-phosphate | mutant BT2/P372A | Zea mays | |
0.06 | - |
alpha-D-glucose 1-phosphate | mutant BT2/A508S | Zea mays | |
0.06 | - |
alpha-D-glucose 1-phosphate | mutant BT2/M172T | Zea mays | |
0.06 | - |
alpha-D-glucose 1-phosphate | mutant BT2/T361C | Zea mays | |
0.06 | - |
alpha-D-glucose 1-phosphate | mutant BT2/V227R | Zea mays | |
0.06 | - |
alpha-D-glucose 1-phosphate | mutant BT2/V502T | Zea mays | |
0.07 | - |
alpha-D-glucose 1-phosphate | mutant BT2/C114A | Zea mays | |
0.07 | - |
alpha-D-glucose 1-phosphate | mutant BT2/D368S | Zea mays | |
0.07 | - |
alpha-D-glucose 1-phosphate | mutant BT2/H149S | Zea mays | |
0.07 | - |
alpha-D-glucose 1-phosphate | mutant BT2/Q213H | Zea mays | |
0.07 | - |
alpha-D-glucose 1-phosphate | wild type BT2/SH2 | Zea mays | |
0.08 | - |
alpha-D-glucose 1-phosphate | mutant BT2/C382F | Zea mays | |
0.09 | - |
ATP | mutant BT2/A508S | Zea mays | |
0.09 | - |
alpha-D-glucose 1-phosphate | mutant BT2/C424V | Zea mays | |
0.09 | - |
ATP | mutant BT2/M172T | Zea mays | |
0.09 | - |
alpha-D-glucose 1-phosphate | mutant BT2/S163F | Zea mays | |
0.11 | - |
ATP | mutant BT2/D368S | Zea mays | |
0.12 | - |
ATP | wild type BT2/SH2 | Zea mays | |
0.13 | - |
ATP | mutant BT2/E438Q | Zea mays | |
0.13 | - |
ATP | mutant BT2/T361C | Zea mays | |
0.14 | - |
ATP | mutant BT2/C114A | Zea mays | |
0.14 | - |
ATP | mutant BT2/C424V | Zea mays | |
0.14 | - |
ATP | mutant BT2/V227R | Zea mays | |
0.15 | - |
ATP | mutant BT2/C382F | Zea mays | |
0.15 | - |
ATP | mutant BT2/V502T | Zea mays | |
0.19 | - |
ATP | mutant BT2/H149S | Zea mays | |
0.19 | - |
ATP | mutant BT2/P372A | Zea mays | |
0.21 | - |
ATP | mutant BT2/Q213H | Zea mays | |
0.42 | - |
ATP | mutant BT2/S163F | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | Solanum tuberosum | - |
diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | Zea mays | - |
diphosphate + ADP-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | Q00081 | fragment | - |
Zea mays | P55241 | - |
- |
Purification (Comment) | Organism |
---|---|
by protamine sulfate and ammonium sulfate fractionation, followed by anion-exchange and affinity chromatography | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Zea mays | - |
tuber | - |
Solanum tuberosum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | diphosphate + ADP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | consists of two small and two large subunits | Solanum tuberosum |
heterotetramer | consists of two small and two large subunits, BT2/SH2 | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
ADP-glucose pyrophosphorylase | - |
Solanum tuberosum |
ADP-glucose pyrophosphorylase | - |
Zea mays |
AGPase | - |
Solanum tuberosum |
AGPase | - |
Zea mays |
BT2 | maize AGPase endosperm small subunit | Zea mays |
SH2 | maize AGPase endosperm large subunit | Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.17 | - |
alpha-D-glucose 1-phosphate | mutant BT2/V227R | Zea mays | |
23.34 | - |
alpha-D-glucose 1-phosphate | mutant BT2/D368S | Zea mays | |
29.36 | - |
alpha-D-glucose 1-phosphate | mutant BT2/Q213H | Zea mays | |
32.87 | - |
alpha-D-glucose 1-phosphate | mutant BT2/E438Q | Zea mays | |
34.65 | - |
alpha-D-glucose 1-phosphate | mutant BT2/S163F | Zea mays | |
35.21 | - |
alpha-D-glucose 1-phosphate | mutant BT2/H149S | Zea mays | |
35.77 | - |
alpha-D-glucose 1-phosphate | mutant BT2/A508S | Zea mays | |
37.21 | - |
alpha-D-glucose 1-phosphate | mutant BT2/C114A | Zea mays | |
38.12 | - |
alpha-D-glucose 1-phosphate | mutant BT2/T361C | Zea mays | |
38.58 | - |
alpha-D-glucose 1-phosphate | mutant BT2/M172T | Zea mays | |
39.17 | - |
alpha-D-glucose 1-phosphate | wild type BT2/SH2 | Zea mays | |
40.75 | - |
alpha-D-glucose 1-phosphate | mutant BT2/C382F | Zea mays | |
41.91 | - |
alpha-D-glucose 1-phosphate | mutant BT2/V502T | Zea mays | |
42.32 | - |
alpha-D-glucose 1-phosphate | mutant BT2/P372A | Zea mays | |
59.86 | - |
alpha-D-glucose 1-phosphate | mutant BT2/C424V | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assay | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Solanum tuberosum | |
ATP | - |
Zea mays |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.83 | - |
phosphate | mutant BT2/S163F, 15 mM 3-phosphoglycerate | Zea mays | |
2.28 | - |
phosphate | mutant BT2/C382F, 15 mM 3-phosphoglycerate | Zea mays | |
3.21 | - |
phosphate | mutant BT2/Q213H, 15 mM 3-phosphoglycerate | Zea mays | |
3.72 | - |
phosphate | mutant BT2/P372A, 15 mM 3-phosphoglycerate | Zea mays | |
3.96 | - |
phosphate | mutant BT2/H149S, 15 mM 3-phosphoglycerate | Zea mays | |
4.26 | - |
phosphate | mutant BT2/D368S, 15 mM 3-phosphoglycerate | Zea mays | |
5.34 | - |
phosphate | mutant BT2/T361C, 15 mM 3-phosphoglycerate | Zea mays | |
13.23 | - |
phosphate | mutant BT2/C114A, 15 mM 3-phosphoglycerate | Zea mays | |
14.5 | - |
phosphate | mutant BT2/V227R, 15 mM 3-phosphoglycerate | Zea mays | |
15.22 | - |
phosphate | mutant BT2/E438Q, 15 mM 3-phosphoglycerate | Zea mays | |
16.8 | - |
phosphate | wild type BT2/SH2, 15 mM 3-phosphoglycerate | Zea mays | |
17.61 | - |
phosphate | mutant BT2/M172T, 15 mM 3-phosphoglycerate | Zea mays | |
17.93 | - |
phosphate | mutant BT2/V502T, 15 mM 3-phosphoglycerate | Zea mays | |
18.36 | - |
phosphate | mutant BT2/C424V, 15 mM 3-phosphoglycerate | Zea mays | |
20.43 | - |
phosphate | mutant BT2/A508S, 15 mM 3-phosphoglycerate | Zea mays |
General Information | Comment | Organism |
---|---|---|
physiological function | ADP-glucose pyrophosphorylase catalyzes the rate-limiting step in starch biosynthesis | Solanum tuberosum |
physiological function | ADP-glucose pyrophosphorylase catalyzes the rate-limiting step in starch biosynthesis | Zea mays |