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Literature summary for 2.7.7.27 extracted from
- Characterization of recombinant UDP- and ADP-glucose pyrophosphorylases and glycogen synthase to elucidate glucose-1-phosphate partitioning into oligo- and polysaccharides in Streptomyces coelicolor (2012), J. Bacteriol., 194, 1485-1493.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 6-phosphate | 2 mM, 21fold activation | Streptomyces coelicolor |  |
| D-glucose 6-phosphate | 2 mM, 33fold activation | Streptomyces coelicolor | |
| D-mannose 6-phosphate | 2 mM, 12fold activation | Streptomyces coelicolor | |
| D-ribose 5-phosphate | 2 mM, 7fold activation | Streptomyces coelicolor | |
| phosphoenolpyruvate | 2 mM, 14fold activation | Streptomyces coelicolor | |
| pyruvate | 2 mM, 3fold activation | Streptomyces coelicolor | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces coelicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Streptomyces coelicolor | |
| phosphate | - |
Streptomyces coelicolor | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
diphosphate | pH 8.0, 37°C | Streptomyces coelicolor | |
| 0.37 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C | Streptomyces coelicolor | |
| 0.39 | - |
ATP | pH 8.0, 37°C | Streptomyces coelicolor | |
| 2.1 | - |
ADP-glucose | pH 8.0, 37°C | Streptomyces coelicolor | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required. Km value in both directions of catalysis about 1.8 mM | Streptomyces coelicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42700 | - |
4 * 42700, calculated and SDS-PAGE | Streptomyces coelicolor |
| 180000 | - |
gel filtration | Streptomyces coelicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | P72394 | - |
- |
| Streptomyces coelicolor A3(2) | P72394 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | enzyme is specific for ATP and reaches similar activities in the directions of ADP-glucose synthesis or diphosphorolysis | Streptomyces coelicolor | diphosphate + ADP-glucose | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | enzyme is specific for ATP and reaches similar activities in the directions of ADP-glucose synthesis or diphosphorolysis | Streptomyces coelicolor A3(2) | diphosphate + ADP-glucose | - |
r | |
| diphosphate + ADP-glucose | enzyme reaches similar activities in the directions of ADP-glucose synthesis or diphosphorolysis | Streptomyces coelicolor | ATP + alpha-D-glucose 1-phosphate | - |
r | |
| diphosphate + ADP-glucose | enzyme reaches similar activities in the directions of ADP-glucose synthesis or diphosphorolysis | Streptomyces coelicolor A3(2) | ATP + alpha-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 42700, calculated and SDS-PAGE | Streptomyces coelicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlgC | - |
Streptomyces coelicolor |
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Release 2023.1 (January 2023)
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