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Literature summary for 2.7.7.4 extracted from
- Purification and properties of ATP-sulfurylase from Furth mouse mastocytoma (1972), Biochim. Biophys. Acta, 258, 113-124.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing inactivates the purified enzyme | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 2 mM | Mus musculus |  |
| Ca2+ | 2 mM | Mus musculus | |
| deoxyadenylylsulfate | 1 mM, in presence of about 20% inhibition | Mus musculus | |
| EDTA | inhibition is reversed by mn2+, Mg2+, Cu2+, Co2+ | Mus musculus | |
| guanylylsulfate | 1 mM, in presence of adenylylsulfate about 20% inhibition | Mus musculus | |
| inosylylsulfate | 1 mM, in presence of adenylylsulfate about 20% inhibition | Mus musculus | |
| Ni2+ | 2 mM | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the reaction does not strictly follow Michaelis-Menten kinetics | Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | no absolute requirement for metal ions, but activity is increased by Mn2+, Mg2+ and Zn2+ | Mus musculus | |
| Mn2+ | no absolute requirement for metal ions, but activity is increased by Mn2+, Mg2+ and Zn2+ | Mus musculus | |
| Zn2+ | no absolute requirement for metal ions, but activity is increased by Mn2+, Mg2+ and Zn2+ | Mus musculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mastocytoma cell | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.3 | - |
- |
Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, purified enzyme solution, 1 mg protein per ml, 15% loss of activity after 5 weeks | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + sulfate | - |
Mus musculus | diphosphate + adenylylsulfate | - |
r | |
| dATP + SO42- | - |
Mus musculus | diphosphate + deoxyadenylylsulfate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Mus musculus |
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Release 2023.1 (January 2023)
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