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Literature summary for 2.7.7.4 extracted from
- Purification and characterization of ATP sulfurylase from the extremely thermophilic archaebacterial sulfate-reducer, Archaeoglobus fulgidus (1990), FEMS Microbiol. Lett., 67, 27-32.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
diphosphate | pH 7.5, 85°C | Archaeoglobus fulgidus |  |
| 0.17 | - |
adenosine 5'-phosphosulfate | pH 7.5, 85°C | Archaeoglobus fulgidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
alpha2beta, 2 * 50000 + 1 * 53000, SDS-PAGE | Archaeoglobus fulgidus |
| 53000 | - |
alpha2beta, 2 * 50000 + 1 * 53000, SDS-PAGE | Archaeoglobus fulgidus |
| 150000 | - |
gel filtration | Archaeoglobus fulgidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Archaeoglobus fulgidus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33.9 | - |
- |
Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + sulfate | - |
Archaeoglobus fulgidus | diphosphate + adenylylsulfate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | alpha2beta, 2 * 50000 + 1 * 53000, SDS-PAGE | Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Archaeoglobus fulgidus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | in Tris-malate and Tris-HCl buffer active between pH 5.5 and pH 9.0 | Archaeoglobus fulgidus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Archaeoglobus fulgidus | isoelectric focusing | 4.4 | 4.3 |
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