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Literature summary for 2.7.7.4 extracted from

  • Gavel, O.Y.; Kladova, A.V.; Bursakov, S.A.; Dias, J.M.; Texeira, S.; Shnyrov, V.L.; Moura, J.J.; Moura, I.; Romao, M.J.; Trincao, J.
    Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 (2008), Acta Crystallogr. Sect. F, 64, 593-595.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Desulfovibrio desulfuricans

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, using 3 M ammonium sulfate, 100 mM Tris-HCl pH 7.5 with 10% (v/v) MPD Desulfovibrio desulfuricans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ contains cobalt Desulfovibrio desulfuricans
Zn2+ contains zinc Desulfovibrio desulfuricans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
-
Desulfovibrio desulfuricans

Organism

Organism UniProt Comment Textmining
Desulfovibrio desulfuricans
-
strain ATCC 27774
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-52 cellulose column chromatography, Source 15Q column chromatography, Superdex 200 gel filtration, and hydroxyapatite column chromatography Desulfovibrio desulfuricans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + sulfate
-
Desulfovibrio desulfuricans diphosphate + adenylylsulfate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 47000 Desulfovibrio desulfuricans

Synonyms

Synonyms Comment Organism
adenosine triphosphate sulfurylase
-
Desulfovibrio desulfuricans
ATPS
-
Desulfovibrio desulfuricans
MgATP:sulfate adenylyltransferase
-
Desulfovibrio desulfuricans