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Literature summary for 2.7.7.40 extracted from
- Bifunctional catalysis by CDP-ribitol synthase: convergent recruitment of the reductase and cytidylyltransferase activities in Haemophilus influenzae and Staphylococcus aureus (2004), Biochemistry, 43, 11802-11812.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of TarI and TarJ individually from their respective genes using the T7 expression system | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CDP-ribitol | competitive versus CTP | Haemophilus influenzae |  |
| CDP-ribitol | competitive versus CTP | Staphylococcus aureus | |
| diphosphate | - |
Haemophilus influenzae | |
| diphosphate | - |
Staphylococcus aureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00851 | - |
CTP | pH 7.2 | Haemophilus influenzae | |
| 0.0147 | - |
D-ribitol 5-phosphate | pH 7.2 | Haemophilus influenzae | |
| 0.0789 | - |
CTP | pH 7.2 | Staphylococcus aureus | |
| 1.28 | - |
D-ribitol 5-phosphate | pH 7.2 | Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
2 * 26000 + 2 * 41000, TarI2J2, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE | Staphylococcus aureus |
| 41000 | - |
2 * 26000 + 2 * 41000, TarI2J2, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE | Staphylococcus aureus |
| 53190 | - |
2 * 53190, calculation from amino acid composition | Haemophilus influenzae |
| 106400 | - |
amino acid composition | Haemophilus influenzae |
| 129000 | - |
calculation from gel filtration and sedimentation velocity data | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemophilus influenzae | - |
- |
- |
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + D-ribitol 5-phosphate | ordered bi-bi mechanism | Haemophilus influenzae | diphosphate + CDP-ribitol | - |
? | |
| CTP + D-ribitol 5-phosphate | tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex. Release and free diffusion of the intermediate ribitol 5-phosphate without channelling between active sites, ordered bi-bi mechanism | Staphylococcus aureus | diphosphate + CDP-ribitol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 2 * 26000 + 2 * 41000, TarI2J2, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE | Staphylococcus aureus |
| ? | 2 * 53190, calculation from amino acid composition | Haemophilus influenzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bcs1 | - |
Haemophilus influenzae |
| cytidylyltransferase | - |
Staphylococcus aureus |
| cytidylyltransferase | - |
Haemophilus influenzae |
| TarIJ | - |
Staphylococcus aureus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
CTP | pH 7.2 | Staphylococcus aureus | |
| 23.5 | - |
CTP | pH 7.2 | Haemophilus influenzae | |
| 76.8 | - |
CTP | pH 7.2 | Staphylococcus aureus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ki(intercept) and Ki(slope)-values | Staphylococcus aureus | |
| additional information | - |
additional information | Ki(intercept) and Ki(slope)-values | Haemophilus influenzae |
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