Activating Compound | Comment | Organism | Structure |
---|---|---|---|
non-ionic detergent | absolute requirement | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CMP | - |
Bacillus subtilis | |
diphosphate | - |
Bacillus subtilis | |
Hg2+ | 0.1 mM, 90% inhibition | Bacillus subtilis | |
Mg2+ | 33% inhibition at 25 mM Mg2+ | Bacillus subtilis | |
PCMB | 0.03 mM, 90% inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
CTP | pH 7.5, 25°C | Bacillus subtilis | |
0.26 | - |
dCTP | pH 7.5, 25°C | Bacillus subtilis | |
0.3 | - |
phosphatidic acid | pH 7.5, 25°C, with 0.45 mM CTP | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | localized exclusively in the membrane fraction | Bacillus subtilis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulating effect increases with concentrations up to 75 mM | Bacillus subtilis | |
Mg2+ | maximal activity with 3-6 mM. 33% inhibition at 25 mM | Bacillus subtilis | |
Mn2+ | 1 mM, 70% of the maximal activity obtained with Mg2+ | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CTP + phosphatidate = diphosphate + CDP-diacylglycerol | ping-pong mechanism | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 1,2-dipalmitoyl phosphatidic acid | - |
Bacillus subtilis | diphosphate + CDP-dipalmitoylglycerol | - |
? | |
CTP + phosphatidate | - |
Bacillus subtilis | diphosphate + CDPdiacylglycerol | - |
? | |
dCTP + phosphatidate | - |
Bacillus subtilis | diphosphate + dCDPdiacylglycerol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
with detergent already inactivated at | Bacillus subtilis |
60 | - |
30 min, membranous enzyme, stable up to | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.7 | pH 6.2: 54% of maximal activity, pH 8.7: 56% of maximal activity | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
diphosphate | pH 7.5, 25°C | Bacillus subtilis | |
5.6 | - |
CMP | pH 7.5, 25°C | Bacillus subtilis |