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Literature summary for 2.7.7.42 extracted from
- ATP: glutamine synthetase adenylyltransferase from Escherichia coli B. Purification and properties (1970), Eur. J. Biochem., 14, 535-544.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-glutamine | highly activating | Escherichia coli |  |
| L-glutamine | activator of adenylylation | Escherichia coli | |
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin, above 1 mg/ml, prevents inactivation at 4°C and 25°C and aggregation | Escherichia coli |
| Mg2+, 20 mM protects to some extent against heat inactivation | Escherichia coli |
| no stabilization by ATP, CTP, Mn2+, glutamine, cysteine or mercaptoethanol each at 20 mM, 2 mM DTT, 20% glycerol, sucrose, polyethyleneglycol or urea at 1 M | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Methyl-L-glutamate | - |
Escherichia coli | |
| 6-diazo-5-oxonorleucine | - |
Escherichia coli | |
| ADP | - |
Escherichia coli | |
| ATP | inhibitory above 1 mM in presence of glutamine, not related to Mg2+ concentration | Escherichia coli | |
| CTP | - |
Escherichia coli | |
| D-glutamine | - |
Escherichia coli | |
| diphosphate | - |
Escherichia coli | |
| DL-2-aminobutyric acid | - |
Escherichia coli | |
| glutamate | L- and D-isomer | Escherichia coli | |
| ITP | - |
Escherichia coli | |
| L-methionine | - |
Escherichia coli | |
| L-tryptophan | - |
Escherichia coli | |
| phosphate | - |
Escherichia coli | |
| S-(2-Hydroxyethyl)-L-cysteine | - |
Escherichia coli | |
| sulfate | - |
Escherichia coli | |
| UTP | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
[L-glutamate:ammonia ligase (ADP-forming)] | pH 7.6, 30°C | Escherichia coli | |
| 0.15 | - |
ATP | pH 7.6, 30°C, in presence of glutamine | Escherichia coli | |
| 0.95 | - |
ATP | pH 7.6, 30°C, in absence of glutamine | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ or Mn2+ required | Escherichia coli | |
| Mg2+ | half-maximal activity at: 14 mM | Escherichia coli | |
| Mn2+ | Mn2+ or Mg2+ required | Escherichia coli | |
| Mn2+ | half-maximal activity at 1.4 mM | Escherichia coli | |
| additional information | Ca2+, Zn2+ and Cu2+ at 10 mM are ineffective | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 145000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [L-glutamate:ammonia ligase (ADP-forming)] | Escherichia coli | - |
diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP) | - |
r | |
| ATP + [L-glutamate:ammonia ligase (ADP-forming)] | Escherichia coli B / ATCC 11303 | - |
diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP) | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
B | - |
| Escherichia coli B / ATCC 11303 | - |
B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 300fold to homogeneity | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [L-glutamate:ammonia ligase (ADP-forming)] | ADP, AMP, cAMP, UTP, CTP, ITP, NAD+ cannot replace ATP | Escherichia coli | diphosphate + [L-glutamate:ammonia ligase (ADP-forming)]-(AMP) | - |
r | |
| ATP + [L-glutamate:ammonia ligase (ADP-forming)] | ADP, AMP, cAMP, UTP, CTP, ITP, NAD+ cannot replace ATP | Escherichia coli B / ATCC 11303 | diphosphate + [L-glutamate:ammonia ligase (ADP-forming)]-(AMP) | - |
r | |
| ATP + [L-glutamate:ammonia ligase (ADP-forming)] | - |
Escherichia coli | diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP) | - |
r | |
| ATP + [L-glutamate:ammonia ligase (ADP-forming)] | - |
Escherichia coli B / ATCC 11303 | diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP) | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 32 | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.8 | pH 5.5: about 50% of activity maximum, pH 9.8: about 35% of activity maximum | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
4°C, 12 h, loss of 70% of initial activity | Escherichia coli |
| 4 | 9 | 4°C, 12 h, no loss of activity | Escherichia coli |
| 9.5 | - |
4°C, 12 h, loss of 50% of initial activity | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | absolutely required | Escherichia coli | |
| ATP | inhibitory at higher comcentrations | Escherichia coli | |
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