Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Gln | C-terminal truncation constructs are dependent on Gln for full adenylylation activity | Escherichia coli | |
Gln | wild-tpye AT needs both signal transduction protein PII and Gln to stimulate full adenylylation activity | Escherichia coli | |
signal transduction protein PII | wild-tpye AT needs both signal transduction protein PII and Gln to stimulate full adenylylation activity | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli DH5alpha, TG1 and JM109 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | all C-terminal truncation constructs are dependent on Gln for full adenylylation activity | Escherichia coli |
additional information | mAbs to AT are used to demonstrate that signal transduction protein PII and signal transduction protein PII-UMP probably bind in the central regulatory domain between residues 466 and 507 to stimulate the adenylylation and deadenylylation reactions, respectively | Escherichia coli |
additional information | solubility and enzymatic analysis of several deletion constructs of AT show that AT has three domains: the two activity domains and a central, probably regulatory, domain connected by interdomain Q-linkers (N-Q1-R-Q2-C), the various constructs which have the opposing domain removed, all retain their activity in the absence of their nitrogen status indicator signal transduction protein PII or signal transduction protein PII-UMP | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylyl-[glutamine synthase] + phosphate | deadenylylation reaction | Escherichia coli | glutamine synthase + ADP | - |
r | |
glutamine synthase + ATP | adenylylation reaction | Escherichia coli | adenylyl-[glutamine synthase] + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
adenylyltransferase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |