Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CTP | above 5 mM | Cricetulus griseus | |
Cu2+ | inhibition in presence of Mg2+ | Cricetulus griseus | |
NEM | - |
Cricetulus griseus | |
Zn2+ | inhibits in presence of Mg2+ | Cricetulus griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
N-acetylneuraminate | pH 9.0, 37°C | Cricetulus griseus | |
1.3 | - |
CTP | pH 9.0, 37°C | Cricetulus griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 10% of the activation with Mg2+ | Cricetulus griseus | |
Mg2+ | maximal activity at 20 mM | Cricetulus griseus | |
Mn2+ | 10% of the activity with Mg2+ | Cricetulus griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | Cricetulus griseus | the lec32 mutation reduces CMP-NeuAc synthetase activity to undetectable levels and reduces Neu-Ac on glycoproteins and glycolipids by 95% | diphosphate + CMP-N-acylneuraminate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | - |
wild-type and glycosylation mutant LEC29.Lec32, the mutation resides in either the structural gene encoding CMP-NeuAc synthetase or in a gene that regulates the prpduction of the active enzyme | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CHO cell | - |
Cricetulus griseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | - |
Cricetulus griseus | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | the lec32 mutation reduces CMP-NeuAc synthetase activity to undetectable levels and reduces Neu-Ac on glycoproteins and glycolipids by 95% | Cricetulus griseus | diphosphate + CMP-N-acylneuraminate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Cricetulus griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reaction with Mn2+ | Cricetulus griseus |
8.5 | 9 | activity in presence of Mg2+ | Cricetulus griseus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | pH 7.5: about 20% of maximal activity, pH 8.0: about 90% of maximal activity, pH 9.5: about 65% of maximal activity | Cricetulus griseus |