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Literature summary for 2.7.7.43 extracted from

  • Potvin, B.; Raju, T.S.; Stanley, P.
    Lec32 is a new mutation in Chinese hamster ovary cells that essentially abrogates CMP-N-acetylneuraminic acid synthetase activity (1995), J. Biol. Chem., 270, 30415-30421.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CTP above 5 mM Cricetulus griseus
Cu2+ inhibition in presence of Mg2+ Cricetulus griseus
NEM
-
Cricetulus griseus
Zn2+ inhibits in presence of Mg2+ Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
N-acetylneuraminate pH 9.0, 37°C Cricetulus griseus
1.3
-
CTP pH 9.0, 37°C Cricetulus griseus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 10% of the activation with Mg2+ Cricetulus griseus
Mg2+ maximal activity at 20 mM Cricetulus griseus
Mn2+ 10% of the activity with Mg2+ Cricetulus griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + N-acylneuraminate Cricetulus griseus the lec32 mutation reduces CMP-NeuAc synthetase activity to undetectable levels and reduces Neu-Ac on glycoproteins and glycolipids by 95% diphosphate + CMP-N-acylneuraminate
-
?

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
wild-type and glycosylation mutant LEC29.Lec32, the mutation resides in either the structural gene encoding CMP-NeuAc synthetase or in a gene that regulates the prpduction of the active enzyme
-

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell
-
Cricetulus griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + N-acylneuraminate
-
Cricetulus griseus diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate the lec32 mutation reduces CMP-NeuAc synthetase activity to undetectable levels and reduces Neu-Ac on glycoproteins and glycolipids by 95% Cricetulus griseus diphosphate + CMP-N-acylneuraminate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Cricetulus griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reaction with Mn2+ Cricetulus griseus
8.5 9 activity in presence of Mg2+ Cricetulus griseus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5 pH 7.5: about 20% of maximal activity, pH 8.0: about 90% of maximal activity, pH 9.5: about 65% of maximal activity Cricetulus griseus