Cloned (Comment) | Organism |
---|---|
DmCSAS is cloned into the baculovirus vector pBlueBac4.5 (pBlueBac-DmCSAS4) which is used to transfect LEC29.Lec32 cells | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | in contrast to its mammalian counterparts DmCSAS protein begins with an N-terminal sequence rich in hydrophobic amino acids characteristic of a signal/anchoring sequence | Drosophila melanogaster |
additional information | multiple alignment among the Drosophila melanogaster, human, mouse, Escherichia coli and Neisseria meningitidis CSAS enzymes shows that all proteins are homologous over the length of the catalytic domain | Drosophila melanogaster |
additional information | replacement of the N-terminal leader sequence of DmCSAS with the human CSAS N-terminal sequence results in the redirection of the chimeric CSAS protein to the nucleus but with concomitant loss of activity | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | unlike all vertebrate CSAS that localize to the nucleus, the DmCSAS is localized to the Golgi compartment | Drosophila melanogaster | 5794 | - |
additional information | replacement of the N-terminal leader sequence of DmCSAS with the human CSAS N-terminal sequence results in the redirection of the chimeric CSAS protein to the nucleus but with concomitant loss of activity | Drosophila melanogaster | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | assay with 20 mM Mg2+ | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
predicted from cDNA | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
head | DmCSAS gene is greatly enriched in adult head, suggesting a possible role in the central nervous system | Drosophila melanogaster | - |
additional information | DmCSAS gene is developmentally regulated, with expression first appearing at 12-24 h of embryogenesis, low expression through larval and pubal stages | Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetylneuraminic acid + CTP | DmCSAS is shown to be functional both in vitro and in vivo | Drosophila melanogaster | CMP-Neu5Ac + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CMP-sialic acid synthetase | - |
Drosophila melanogaster |
DmCSAS | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Drosophila melanogaster |