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Literature summary for 2.7.7.43 extracted from

  • Mizanur, R.M.; Pohl, N.L.
    Bacterial CMP-sialic acid synthetases: production, properties, and applications (2008), Appl. Microbiol. Biotechnol., 80, 757-765.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no enhancement of activity with dithiothreitol, glutathione, or beta-mercaptoethanol Mannheimia haemolytica

General Stability

General Stability Organism
the enzyme is equally stable with or without the presence of glycerol at different temperatures Acetivibrio thermocellus

Inhibitors

Inhibitors Comment Organism Structure
5-mercury-CTP
-
Escherichia coli
EDTA treatment with EDTA results in loss of catalytic activity which can be restored by the addition of either Mg2+ or Mn2+ Escherichia coli
additional information dithiothreitol has little effect on enzyme activity [Haemophilus] ducreyi
p-hydroxymercuribenzoic acid
-
Mannheimia haemolytica
periodate-oxidized CTP
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
CTP at pH 7.1 and 37°C [Haemophilus] ducreyi
0.13
-
N-acylneuraminate
-
Acetivibrio thermocellus
0.24
-
CTP
-
Acetivibrio thermocellus
0.26
-
N-acylneuraminate at pH 7.1 and 37°C [Haemophilus] ducreyi
0.31
-
CTP
-
Neisseria meningitidis
0.31
-
CTP at 37°C Escherichia coli
0.34
-
N-acylneuraminate
-
Neisseria meningitidis
1.77
-
CTP
-
Mannheimia haemolytica
1.82
-
N-acylneuraminate
-
Mannheimia haemolytica
4
-
N-acylneuraminate at 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ partially substitutes Mg2+ in catalysis Mannheimia haemolytica
Co2+ partially substitutes Mg2+ in catalysis Mannheimia haemolytica
Mg2+ required for activity Neisseria meningitidis
Mg2+ absolutely required for activity, 20 mM Mg2+ gives the most activity [Haemophilus] ducreyi
Mg2+ required for activity, 20-40 mM Mg2+ is the optimum concentration Escherichia coli
Mg2+ required for activity, maximum activity at 10 mM Mg2+, decreasing at higher Mg2+ concentrations Mannheimia haemolytica
Mn2+ required for activity Neisseria meningitidis
Mn2+ partially substitutes Mg2+ in catalysis Mannheimia haemolytica
Mn2+ required for activity, can be replaced by Mg2+, Co2+, and Zn2+ in decreasing order Acetivibrio thermocellus
Mn2+ required for activity, Mn2+ is only 40% as effective as Mg2+ at 20 mM [Haemophilus] ducreyi
additional information Ca2+ shows no effect on enzyme activity [Haemophilus] ducreyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24800
-
gel filtration Neisseria meningitidis
25400
-
gel filtration [Haemophilus] ducreyi
26000
-
SDS-PAGE Acetivibrio thermocellus
43000
-
SDS-PAGE Mannheimia haemolytica
49000
-
SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + N-acylneuraminate Escherichia coli
-
diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate Neisseria meningitidis
-
diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate Mannheimia haemolytica
-
diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate Acetivibrio thermocellus
-
diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate [Haemophilus] ducreyi
-
diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate Escherichia coli K-235
-
diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate Mannheimia haemolytica A2
-
diphosphate + CMP-N-acylneuraminate
-
?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Escherichia coli
-
-
-
Escherichia coli K-235
-
-
-
Mannheimia haemolytica
-
-
-
Mannheimia haemolytica A2
-
-
-
Neisseria meningitidis
-
-
-
[Haemophilus] ducreyi
-
-
-

Storage Stability

Storage Stability Organism
-20°C or -80°C, in 50% (v/v) glycerol, several months, remains stable [Haemophilus] ducreyi
4°C, at pH 7.0, several weeks, remains stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP + N-acetylneuraminate
-
Mannheimia haemolytica phosphate + CMP-N-acetylneuraminate
-
?
CDP + N-acetylneuraminate
-
Mannheimia haemolytica A2 phosphate + CMP-N-acetylneuraminate
-
?
CTP + (N-4-O-)diacetylneuraminic acid (N-4-O-)diacetylneuraminic acid is 46% effective compared to N-acylneuraminate Neisseria meningitidis diphosphate + CMP-N-4-O-diacetylneuraminate
-
?
CTP + N-acylneuraminate
-
Escherichia coli diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate
-
Neisseria meningitidis diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate
-
Mannheimia haemolytica diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate
-
Acetivibrio thermocellus diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate
-
[Haemophilus] ducreyi diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate
-
Escherichia coli K-235 diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate
-
Mannheimia haemolytica A2 diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-glycolylneuraminic acid
-
Mannheimia haemolytica CMP-N-glycolylneuraminate + diphosphate
-
?
CTP + N-glycolylneuraminic acid
-
Acetivibrio thermocellus CMP-N-glycolylneuraminate + diphosphate
-
?
CTP + N-glycolylneuraminic acid
-
[Haemophilus] ducreyi CMP-N-glycolylneuraminate + diphosphate
-
?
CTP + N-glycolylneuraminic acid
-
Mannheimia haemolytica A2 CMP-N-glycolylneuraminate + diphosphate
-
?
additional information does not accept N-glycolylneuraminic acid and triacetylneuraminic acid as substrates Neisseria meningitidis ?
-
?
additional information does not utilize N-glycolylneuraminic acid as a substrate Escherichia coli ?
-
?
additional information does not utilize N-glycolylneuraminic acid as a substrate Escherichia coli K-235 ?
-
?
TTP + N-glycolylneuraminic acid
-
Mannheimia haemolytica TMP-N-glycolylneuraminate + diphosphate
-
?
TTP + N-glycolylneuraminic acid
-
Mannheimia haemolytica A2 TMP-N-glycolylneuraminate + diphosphate
-
?
UDP + N-acylneuraminate
-
Mannheimia haemolytica phosphate + UMP-N-acetylneuraminate
-
?
UDP + N-acylneuraminate
-
Mannheimia haemolytica A2 phosphate + UMP-N-acetylneuraminate
-
?
UTP + N-glycolylneuraminic acid
-
Mannheimia haemolytica UMP-N-glycolylneuraminate + diphosphate
-
?

Synonyms

Synonyms Comment Organism
CMP-N-acetylneuraminic acid synthetase
-
Escherichia coli
CMP-N-acetylneuraminic acid synthetase
-
Neisseria meningitidis
CMP-N-acetylneuraminic acid synthetase
-
Mannheimia haemolytica
CMP-N-acetylneuraminic acid synthetase
-
Acetivibrio thermocellus
CMP-N-acetylneuraminic acid synthetase
-
[Haemophilus] ducreyi
CSS
-
Escherichia coli
CSS
-
Neisseria meningitidis
CSS
-
Mannheimia haemolytica
CSS
-
Acetivibrio thermocellus
CSS
-
[Haemophilus] ducreyi
cytidine 5'-monophosphate N-acetylneuraminic acid synthetase
-
Escherichia coli
cytidine 5'-monophosphate N-acetylneuraminic acid synthetase
-
Neisseria meningitidis
cytidine 5'-monophosphate N-acetylneuraminic acid synthetase
-
Mannheimia haemolytica
cytidine 5'-monophosphate N-acetylneuraminic acid synthetase
-
Acetivibrio thermocellus
cytidine 5'-monophosphate N-acetylneuraminic acid synthetase
-
[Haemophilus] ducreyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Escherichia coli
37
-
-
Neisseria meningitidis
37
-
-
[Haemophilus] ducreyi
50
-
-
Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 45 the enzyme loses complete activity after 50 and 10 min when stored at 40°C and 45°C, respectively Escherichia coli
70
-
the enzyme retains activity at 70°C Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9.5
-
[Haemophilus] ducreyi
8.5
-
-
Neisseria meningitidis
9
-
-
Mannheimia haemolytica
9 10
-
Escherichia coli
9.5
-
-
Acetivibrio thermocellus

pH Range

pH Minimum pH Maximum Comment Organism
7 13
-
Acetivibrio thermocellus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 12.5 activity is drastically reduced below pH 7.0 and above pH 12.5 Mannheimia haemolytica