Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no necessity of including a sulfhydryl reagent, such as dithiothreitol, in the assay reaction | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
D209A | almost complete loss of activity. Residue involved in metal binding | Neisseria meningitidis |
D211A | dramatic loss of activity. Residue involved in metal binding | Neisseria meningitidis |
F192A | 8fold increase on Km value for CTP | Neisseria meningitidis |
F193A | 3fold increase on Km value for CTP | Neisseria meningitidis |
K142A | 10000fold reduction in kcat with an insignificant, fourfold, rise in Km for CTP | Neisseria meningitidis |
K142A | dramatic loss of activity. Residue involved in metal binding | Neisseria meningitidis |
N175A | 16fold increases in the Km value for CTP and 23fold for N-acetylneuraminate | Neisseria meningitidis |
Q104A | 40fold inccrease in Km value for N-acetylneuraminate | Neisseria meningitidis |
Q104E | dramatic loss of activity. Residue involved in metal binding | Neisseria meningitidis |
Q104L | dramatic loss of activity. Residue involved in metal binding | Neisseria meningitidis |
Q104N | dramatic loss of activity. Residue involved in metal binding | Neisseria meningitidis |
R173A | 38fold increase in Km value for N-acetylneuraminate | Neisseria meningitidis |
Y179A | 200fold decrease in kcat value | Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
CTP | wild-type, pH 7.5, 22°C | Neisseria meningitidis | |
0.026 | - |
CTP | mutant D211A, pH 7.5, 22°C | Neisseria meningitidis | |
0.031 | - |
CTP | mutant Q104A, pH 7.5, 22°C | Neisseria meningitidis | |
0.04 | - |
CTP | mutant Y179A, pH 7.5, 22°C | Neisseria meningitidis | |
0.048 | - |
CTP | mutant R173A, pH 7.5, 22°C | Neisseria meningitidis | |
0.052 | - |
CTP | mutant F193A, pH 7.5, 22°C | Neisseria meningitidis | |
0.066 | - |
N-acetylneuraminate | mutant D211A, pH 7.5, 22°C | Neisseria meningitidis | |
0.068 | - |
N-acetylneuraminate | wild-type, pH 7.5, 22°C | Neisseria meningitidis | |
0.11 | - |
N-acetylneuraminate | mutant Y179A, pH 7.5, 22°C | Neisseria meningitidis | |
0.15 | - |
CTP | mutant F192A, pH 7.5, 22°C | Neisseria meningitidis | |
0.27 | - |
CTP | mutant N175A, pH 7.5, 22°C | Neisseria meningitidis | |
0.29 | - |
N-acetylneuraminate | mutant R173A, pH 7.5, 22°C | Neisseria meningitidis | |
0.38 | - |
N-acetylneuraminate | mutant F192A, pH 7.5, 22°C | Neisseria meningitidis | |
0.78 | - |
N-acetylneuraminate | mutant F193A, pH 7.5, 22°C | Neisseria meningitidis | |
1.6 | - |
N-acetylneuraminate | mutant N175A, pH 7.5, 22°C | Neisseria meningitidis | |
2.6 | - |
N-acetylneuraminate | mutant Q104A, pH 7.5, 22°C | Neisseria meningitidis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enzyme recruits two Mg2+ ions during the catalytic cycle | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | P0A0Z8 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CTP + N-acetylneuraminate = diphosphate + CMP-N-acylneuraminate | typical ordered-sequential kinetic behaviour, enzyme does not bind N-acetylneuraminate in the absence of CTP | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acetylneuraminate | - |
Neisseria meningitidis | diphosphate + CMP-N-acetylneuraminate | - |
? | |
additional information | no substrate: 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid, kcat/Km value is about 5000fold lower than that of N-acetylneuraminate | Neisseria meningitidis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CMP-Neu5Ac synthetase | - |
Neisseria meningitidis |
CNS | - |
Neisseria meningitidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
CTP | mutant D211A, pH 7.5, 22°C | Neisseria meningitidis | |
0.05 | - |
CTP | mutant Y179A, pH 7.5, 22°C | Neisseria meningitidis | |
2.2 | - |
CTP | mutant Q104A, pH 7.5, 22°C | Neisseria meningitidis | |
5 | - |
CTP | mutant F193A, pH 7.5, 22°C | Neisseria meningitidis | |
7 | - |
CTP | mutant R173A, pH 7.5, 22°C | Neisseria meningitidis | |
10.2 | - |
CTP | wild-type, pH 7.5, 22°C | Neisseria meningitidis | |
20 | - |
CTP | mutant F192A, pH 7.5, 22°C | Neisseria meningitidis | |
25 | - |
CTP | mutant N175A, pH 7.5, 22°C | Neisseria meningitidis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
N-acetylneuraminate | mutant Y179A, pH 7.5, 22°C | Neisseria meningitidis | |
0.0009 | - |
N-acetylneuraminate | mutant Q104A, pH 7.5, 22°C | Neisseria meningitidis | |
0.0013 | - |
CTP | mutant Y179A, pH 7.5, 22°C | Neisseria meningitidis | |
0.0063 | - |
N-acetylneuraminate | mutant F193A, pH 7.5, 22°C | Neisseria meningitidis | |
0.052 | - |
N-acetylneuraminate | mutant F192A, pH 7.5, 22°C | Neisseria meningitidis | |
0.072 | - |
CTP | mutant Q104A, pH 7.5, 22°C | Neisseria meningitidis | |
0.09 | - |
CTP | mutant N175A, pH 7.5, 22°C | Neisseria meningitidis | |
0.097 | - |
CTP | mutant F193A, pH 7.5, 22°C | Neisseria meningitidis | |
0.132 | - |
CTP | mutant F192A, pH 7.5, 22°C | Neisseria meningitidis | |
0.143 | - |
CTP | mutant R173A, pH 7.5, 22°C | Neisseria meningitidis | |
0.148 | - |
N-acetylneuraminate | wild-type, pH 7.5, 22°C | Neisseria meningitidis | |
0.155 | - |
N-acetylneuraminate | mutant N175A, pH 7.5, 22°C | Neisseria meningitidis | |
0.23 | - |
N-acetylneuraminate | mutant R173A, pH 7.5, 22°C | Neisseria meningitidis | |
0.6 | - |
CTP | wild-type, pH 7.5, 22°C | Neisseria meningitidis |