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Literature summary for 2.7.7.48 extracted from
- Extensive purification of the cucumber mosaic virus-induced RNA replicase (1979), Virology, 96, 622-632.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Cucumber mosaic virus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
a major polypeptide of 100000 Da is specific for the RNA replicase, determined by SDS-PAGE | Cucumber mosaic virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumber mosaic virus | - |
Cucumis sativus seedlings infected with | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cucumber mosaic virus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.012 | - |
activity with CMV RNA | Cucumber mosaic virus |
| 0.13 | - |
activity with poly(C) | Cucumber mosaic virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | the enzyme copies CMV RNA and several other viral RNAs, Brome mosaic virus RNA, Alfalfa mosaic virus RNA and Tobacco mosaic virus RNA. Activity with poly(C) and poly(U) but not poly(A) or poly(G). The product with CMV RNA as template is heterogenous in size with a peak length of about 150 residues | Cucumber mosaic virus | diphosphate + RNAn+1 | - |
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