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Literature summary for 2.7.7.48 extracted from

  • Yonesaki, T.; Haruna, I.
    In vitro replication of Bacteriophage GA RNA. Subunit structure and catalytic properties of GA replicase (1981), J. Biochem., 89, 741-750.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information activity is lowered at ionic strength of 0.1 Enterobacteria phage GA
RNase RNAse A, 0.01 mg/ml, or Rnase T1, 0.002 mg/ml, inhibit the reaction by 90-95% Enterobacteria phage GA

Organism

Organism UniProt Comment Textmining
Enterobacteria phage GA
-
Escherichia coli infected with
-

Purification (Commentary)

Purification (Comment) Organism
-
Enterobacteria phage GA

Storage Stability

Storage Stability Organism
-20°C, 25% glycerol, half-life is 2 months Enterobacteria phage GA
0°C, 25% glycerol, half-life is 10 d Enterobacteria phage GA

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn
-
Enterobacteria phage GA diphosphate + RNAn+1
-
?

Subunits

Subunits Comment Organism
More the enzyme contains four different subunits: I, II, II and IV. The molecular weights of which are 74000 Da, 60000 Da, 47000 Da and 36000 Da, respectively. Three of them, I, III and IV are host-coded proteins, ribosomal protein S1 and elongation factor Tu(III) and Ts(IV) Enterobacteria phage GA