Protein Variants | Comment | Organism |
---|---|---|
D225A | mutant enzyme is capable of robust de novo initiation in the presence of Mn2+, produces reduced amounts of product of 38 nt and longer that arose from template switch | Hepacivirus C |
R158A | mutation decreases de novo initiation of RNA synthesis | Hepacivirus C |
R158A/R367A/R386A | mutation eliminates de novo initiation product even in presence of Mn2+, but enzyme is capable of primer extension | Hepacivirus C |
R158A/R386A | mutation eliminates de novo initiation product even in presence of Mn2+, but enzyme is capable of primer extension | Hepacivirus C |
R158A/R394A | mutation eliminates de novo initiation product even in presence of Mn2+, but enzyme is capable of primer extension | Hepacivirus C |
R386A | mutation decreases de novo initiation of RNA synthesis | Hepacivirus C |
R394A | mutation decreases de novo initiation of RNA synthesis | Hepacivirus C |
R48A | mutation decreases de novo initiation of RNA synthesis | Hepacivirus C |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
GTP | pH 8.2, 25°C, enzyme form DELTA21 that lacks the C-terminal 21 hydrophobic residues | Hepacivirus C | |
5.05 | - |
GTP | pH 8.2, 25°C, mutant enzyme | Hepacivirus C | |
10.1 | - |
GTP | pH 8.2, 25°C, mutant enzyme | Hepacivirus C | |
11.25 | - |
GTP | pH 8.2, 25°C, mutant enzyme | Hepacivirus C | |
15.3 | - |
GTP | pH 8.2, 25°C, mutant enzyme R158A | Hepacivirus C | |
25.9 | - |
GTP | pH 8.2, 25°C, mutant enzyme | Hepacivirus C | |
33.8 | - |
GTP | pH 8.2, 25°C, mutant enzyme | Hepacivirus C |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | likely helps to stabilize the interaction between the active site and the NTPi | Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
expression in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
enzyme expressed in Escherichia coli | Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NS5B | - |
Hepacivirus C |
RNA-dependent RNA polymerase | - |
Hepacivirus C |