Cloned (Comment) | Organism |
---|---|
fusion protein bearing a His6 tag at its C-terminus or at its N terminus is overexpressed in Escherichia coli | Sapovirus |
Crystallization (Comment) | Organism |
---|---|
overall structure of the sapovirus 3Dpol is determined by X-ray crystallography to 2.32 A resolution | Sapovirus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | over a concentration range from 0.5 to 5 mM, sapovirus 3Dpol displays a clear preference for Mn2+ over Mg2+ | Sapovirus | |
Mn2+ | over a concentration range from 0.5 to 5 mM, sapovirus 3Dpol displays a clear preference for Mn2+ over Mg2+ | Sapovirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Sapovirus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | Sapovirus | key enzyme of replication | diphosphate + RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sapovirus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | key enzyme of replication | Sapovirus | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | in a synthetic RNA template-dependent reaction, sapovirus 3Dpol synthesizes a double-stranded RNA or labels the template 3' terminus by terminal transferase activity. Initiation of RNA synthesis occurs de novo on heteropolymeric templates or in a primer dependent manner on polyadenylated templates | Sapovirus | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Sapovirus |
Synonyms | Comment | Organism |
---|---|---|
3Dpol-like protein | the RNA-dependent RNA polymerase is associated with the 3Dpol-like protein | Sapovirus |