Literature summary for 2.7.7.48 extracted from

Kim, S.J.; Kim, J.H.; Sun, J.M.; Kim, M.G.; Oh, J.W.
Suppression of hepatitis C virus replication by protein kinase C-related kinase 2 inhibitors that block phosphorylation of viral RNA polymerase (2009), J. Viral Hepat., 16, 697-704.

Search for more literature for 2.7.7.48

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	RdRp phosphorylation by PRK2 is required for activation and RNA replication	Hepacivirus C

Cloned(Commentary)

Cloned (Comment)	Organism
expression of NS5B in Huh7 cells	Hepacivirus C

Inhibitors

Inhibitors	Comment	Organism	Structure
HA1077	i.e. fasudil, reduces the levels of intracellular viral RNA by 70% at 0.02 mM, the RdRp activity of HCV NS5B is not inhibited by the PRK2 inhibitor, but its activation by phosphorylation through PRK2	Hepacivirus C
additional information	PRK2 inhibitors can be antiviral drugs that act by suppressing HCV replication via inhibition of viral RNA polymerase phosphorylation	Hepacivirus C
Y27632	blocks HCV RdRp phosphorylation by suppressing PRK2 activation, reduces the levels of intracellular viral RNA by 92% at 0.02 mM, the RdRp activity of HCV NS5B is not inhibited by the PRK2 inhibitor, but its activation by phosphorylation through PRK2	Hepacivirus C

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Hepacivirus C

Organism

Organism	UniProt	Comment	Textmining
Hepacivirus C	-	i.e. HCV	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	RdRp phosphorylation by PRK2 is required for activation and RNA replication	Hepacivirus C

Synonyms

Synonyms	Comment	Organism
nonstructural protein 5B	-	Hepacivirus C
NS5B	-	Hepacivirus C
RDRP	-	Hepacivirus C
RNA-dependent RNA polymerase	-	Hepacivirus C

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
28	-	assay at	Hepacivirus C

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Hepacivirus C

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Release 2023.1 (January 2023)