Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | to observe full activity of the enzyme, it is necessary to treat the virions with a non-ionic detergent. If the treatment is at 40°C the presence of dithiothreitol is necessary to recover activity | Rous sarcoma virus | |
Nonionic detergent | to observe full activity of the enzyme, it is necessary to treat the virions with a non-ionic detergent. If the treatment is at 40°C the presence of dithiothreitol is necessary to recover activity | Rous sarcoma virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 80 mM, 60% inhibition | Rous sarcoma virus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | monovalent cation is not required for activity, 20 mM KCl causes 15% stimulation | Rous sarcoma virus | |
Mg2+ | absolute requirement, optimal concentration: 10 mM | Rous sarcoma virus | |
Mn2+ | MnCl2 can substitute for MgCl2 | Rous sarcoma virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rous sarcoma virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | all four deoxyribonucleotide triphosphates are required for full activity, some activity is present when only three deoxyribonucleotide triphosphates are added and 10-20% of full activity is still present with only two deoxyribonucleotide triphosphates | Rous sarcoma virus | diphosphate + DNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | - |
Rous sarcoma virus |