Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Escherichia coli |
67227 | - |
x * 67227, calculation from nucleotide sequence | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Myxococcus xanthus | - |
- |
- |
Stigmatella aurantiaca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
it is important to purify RT-Ec67 from an Escherichia coli strain defective in DNA polymerase I, because this enzyme can utilize an RNA template to synthesize DNA | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Myxococcus xanthus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Stigmatella aurantiaca | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the purified enzyme can synthesize DNA using RNA as a template and a synthetic oligodeoxynucleotide as a primer: cDNA can be synthesized using the Escherichi coli 5S RNA as template and a 15-base synthetic oligonucleotide complementary to the 3'-end of the 5S RNA as a primer. The enzyme can also produce a full-length cDNA using a 50-base synthetic DNA as a template and a synthetic oligonucleotide complementary to the 3'-end of the template as a primer | Escherichia coli | diphosphate + DNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Escherichia coli |
? | x * 67227, calculation from nucleotide sequence | Escherichia coli |