Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 with 10 mM dithiothreitol | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
human LINE-1 ORF2, which encodes reverse transcriptase, is inserted into a baculovirus shuttle vector and expressed in SF21 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | above 1 mM MnCl2 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 with 50 mM KCl | Homo sapiens | |
Mg2+ | divalent metal ion required, enzyme has a preference for Mg2+ over Mn2+. Activity on poly(rA)*oligo(dT)12 is almost 3fold higher with Mg2+ than with Mn2+ | Homo sapiens | |
Mn2+ | divalent metal ion required, enzyme has a preference for Mg2+ over Mn2+. Activity on poly(rA)*oligo(dT)12 is almost 3fold higher with Mg2+ than with Mn2+. Maximal dTTP incorporation into poly(rA)*oligo(dT)12 occurs between 0.3 and 0.6 mM MnCl2 | Homo sapiens | |
NaCl | maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 at 100 mM NaCl | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human LINE-1 ORF2, which encodes reverse transcriptase, is inserted into a baculovirus shuttle vector and expressed in SF21 cells | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, 50% glycerol, stable for more than 12 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | DNA synthesis of the recombinant enzyme is higher on poly(rA)*oligo(dT)12 than on poly(rC)*oligo(dG). The activity on poly[d(A-T)] is noticeably lower than that on poly(rA)*oligo(dT)12 | Homo sapiens | diphosphate + DNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 47 | 23°C: about 30% of maximal activity, 47°C: about 15% of maximal activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 | Homo sapiens |