Literature summary for 2.7.7.49 extracted from

Kerr, S.G.; Anderson, K.S.
Pre-steady-state kinetic characterization of wild type and 3'-azido-3'-deoxythymidine (AZT) resistant human immunodeficiency virus type 1 reverse transcriptase: implication of RNA directed DNA polymerization in the mechanism of AZT resistance (1997), Biochemistry, 36, 14064-14070.

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Protein Variants

Protein Variants	Comment	Organism
D67N/K70R/T215Y/K219Q	mutation results in a 1.5fold decrease in the rate constant for polymerization and a 2.5fold decrease in the equilibrium dissociation constant for 3'-azido-3'-deoxythymidine 5'-triphosphate compared to wild-type enzyme. These values translate into a 4fold decrease in selectivity for 3'-azido-3'-deoxythymidine 5'-monophosphate incorporation ba the mutant enzyme as compared to wild-type enzyme for RNA dependent DNA replication. No such decrease in selectivity is detected for DNA dependent replication	Human immunodeficiency virus 1

Organism

Organism	UniProt	Comment	Textmining
Avian myeloblastosis virus	-	wild-type and 3'-azido-3'-deoxythymidine resistant strain D67N/K70R/T215Y/K219Q	-
Human immunodeficiency virus 1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and 3'-azido-3'-deoxythymidine resistant strain D67N/K70R/T215Y/K219Q, expression in Escherichia coli	Human immunodeficiency virus 1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deoxynucleoside triphosphate + DNAn	-	Avian myeloblastosis virus	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	-	Human immunodeficiency virus 1	diphosphate + DNAn+1	-	?

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Release 2023.1 (January 2023)