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Literature summary for 2.7.7.49 extracted from

  • Choi, J.Y.; Guengerich, F.P.
    Analysis of the effect of bulk at N2-alkylguanine DNA adducts on catalytic efficiency and fidelity of the processive DNA polymerases bacteriophage T7 exonuclease- and HIV-1 reverse transcriptase (2004), J. Biol. Chem., 279, 19217-19229.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
N2-benzylguanine the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity Human immunodeficiency virus 1
N2-ethylguanine the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity Human immunodeficiency virus 1
N2-isobutylguanine the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity Human immunodeficiency virus 1
N2-methyl(9-anthracenyl)guanine the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity Human immunodeficiency virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00002
-
dCTP
-
Human immunodeficiency virus 1
0.25
-
dTTP
-
Human immunodeficiency virus 1
0.85
-
dATP
-
Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dCTP + DNAn
-
Human immunodeficiency virus 1 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Human immunodeficiency virus 1 diphosphate + DNAn+1
-
?
dTTP + poly(rA)/(dT)18
-
Human immunodeficiency virus 1 ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Human immunodeficiency virus 1