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Literature summary for 2.7.7.49 extracted from
- Comparison of DNA polymerase activities between recombinant feline immunodeficiency and leukemia virus reverse transcriptases (2005), Virology, 335, 106-121.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
feline leukemia virus |
- |
feline immunodeficiency virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3'-azido-3'deoxythymidine 5'-triphosphate | IC50: 0.042 mM | feline immunodeficiency virus |  |
| 3'-azido-3'deoxythymidine 5'-triphosphate | IC50: 0.06 mM | feline leukemia virus | |
| ddATP | 0.015 mM | feline immunodeficiency virus | |
| ddATP | 0.28 mM | feline leukemia virus | |
| lamivudine triphosphate | - |
feline immunodeficiency virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000028 | - |
dCTP | - |
feline immunodeficiency virus | |
| 0.000051 | - |
dTTP | - |
feline immunodeficiency virus | |
| 0.00006 | - |
dGTP | - |
feline immunodeficiency virus | |
| 0.000081 | - |
dATP | - |
feline immunodeficiency virus | |
| 0.000936 | - |
dGTP | - |
feline leukemia virus | |
| 0.00148 | - |
dCTP | - |
feline leukemia virus | |
| 0.00255 | - |
dTTP | - |
feline leukemia virus | |
| 0.004674 | - |
dATP | - |
feline leukemia virus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM, enzyme shows high activity in presence of | feline immunodeficiency virus | |
| Mn2+ | enzyme can effectively use Mn2+ at 0.05 mM | feline immunodeficiency virus | |
| Mn2+ | enzyme can effectively use Mn2+ at 0.05 mM, enzyme does not efficiently use Mg2+ | feline leukemia virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| feline immunodeficiency virus | - |
- |
- |
| feline leukemia virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
feline leukemia virus |
- |
feline immunodeficiency virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dATP + DNAn | - |
feline leukemia virus | diphosphate + DNAn+1 | - |
? | |
| dATP + DNAn | - |
feline immunodeficiency virus | diphosphate + DNAn+1 | - |
? | |
| dCTP + DNAn | - |
feline leukemia virus | diphosphate + DNAn+1 | - |
? | |
| dCTP + DNAn | - |
feline immunodeficiency virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
feline leukemia virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
feline immunodeficiency virus | diphosphate + DNAn+1 | - |
? | |
| dGTP + DNAn | - |
feline leukemia virus | diphosphate + DNAn+1 | - |
? | |
| dGTP + DNAn | - |
feline immunodeficiency virus | diphosphate + DNAn+1 | - |
? | |
| dTTP + poly(rA)/(dT)18 | - |
feline leukemia virus | ? | - |
? | |
| dTTP + poly(rA)/(dT)18 | - |
feline immunodeficiency virus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FeLV RT | - |
feline leukemia virus |
| FIV RT | - |
feline immunodeficiency virus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
dCTP | - |
feline leukemia virus | |
| 0.17 | - |
dTTP | - |
feline leukemia virus | |
| 0.18 | - |
dATP | - |
feline leukemia virus | |
| 0.21 | - |
dATP | - |
feline immunodeficiency virus | |
| 0.22 | - |
dCTP | - |
feline immunodeficiency virus | |
| 0.28 | - |
dTTP | - |
feline immunodeficiency virus | |
| 0.51 | - |
dGTP | - |
feline immunodeficiency virus | |
| 0.56 | - |
dGTP | - |
feline leukemia virus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.042 | - |
IC50: 0.042 mM | feline immunodeficiency virus | 3'-azido-3'deoxythymidine 5'-triphosphate | |
| 0.06 | - |
IC50: 0.06 mM | feline leukemia virus | 3'-azido-3'deoxythymidine 5'-triphosphate | |
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