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Literature summary for 2.7.7.49 extracted from

  • Liu, S.; Goff, S.P.; Gao, G.
    Gln(84) of moloney murine leukemia virus reverse transcriptase regulates the incorporation rates of ribonucleotides and deoxyribonucleotides (2006), FEBS Lett., 580, 1497-1501.
    View publication on PubMed
Search for more literature for 2.7.7.49

Cloned(Commentary)

Cloned (Comment) Organism
recombinantly expressed in Escherichia coli Moloney murine leukemia virus

Protein Variants

Protein Variants Comment Organism
F155V the mutation increase the affinity of the enzyme for ribonucleotides, without affecting the Vmax for catalysis, and thereby conferrs to the enzyme significant RNA polymerase activity Moloney murine leukemia virus
Q84A mutant enzyme displays higher DNA polymerase activities than wild-type enzyme. Vmax of Q84AH using dTTP as a substrate is about three times that of wild-type value, whereas the Km values are very similar Moloney murine leukemia virus
Q84A/F155V Q84A mutation further improves RNA polymerase and DNA polymerase activity of mutant F155V Moloney murine leukemia virus
Q84N mutant enzyme displays higher DNA polymerase activities than wild-type enzyme Moloney murine leukemia virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00503
-
 poly(rA)/(dT)18 mutant enzyme Q84A Moloney murine leukemia virus
0.01005
-
 dTTP mutant enzyme Q84A Moloney murine leukemia virus
0.01137
-
 dTTP mutant enzyme Q84A Moloney murine leukemia virus
0.01171
-
 poly(rA)/(dT)18 wild-type enzyme Moloney murine leukemia virus
0.01294
-
 dTTP mutant enzyme F155H Moloney murine leukemia virus
0.01304
-
 dTTP wild-type enzyme Moloney murine leukemia virus

Organism

Organism UniProt Comment Textmining
Moloney murine leukemia virus P03355
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Moloney murine leukemia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn wild-type Moloney murine leukemia virus reverse transcriptase selectively uses deoxyribonucleotides over ribonucleotides as substrates Moloney murine leukemia virus diphosphate + DNAn+1
-
 ?
dTTP + poly(rA)/(dT)18
-
 Moloney murine leukemia virus ?
-
 ?