Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.49 extracted from

  • Oz-Gleenberg, I.; Herzig, E.; Voronin, N.; Hizi, A.
    Substrate variations that affect the nucleic acid clamp activity of reverse transcriptases (2012), FEBS J., 279, 1894-1903.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn murine leukemia virus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn bovine immunodeficiency virus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
bovine immunodeficiency virus
-
-
-
Human immunodeficiency virus 1
-
-
-
murine leukemia virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
murine leukemia virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Human immunodeficiency virus 1 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
bovine immunodeficiency virus diphosphate + DNAn+1
-
?
additional information reverse transcriptases perform template switches when there is a very short (two-nucleotide) complementarity between the 3' ends of the primer (donor) strand and the DNA or RNA template acceptor strands. Combined two-step clamp/DNA polymerase activity, where the initial clamp is followed by DNA synthesis, overview murine leukemia virus ?
-
?
additional information reverse transcriptases perform template switches when there is a very short (two-nucleotide) complementarity between the 3' ends of the primer (donor) strand and the DNA or RNA template acceptor strands. Combined two-step clamp/DNA polymerase activity, where the initial clamp is followed by DNA synthesis, overview Human immunodeficiency virus 1 ?
-
?
additional information reverse transcriptases perform template switches when there is a very short (two-nucleotide) complementarity between the 3' ends of the primer (donor) strand and the DNA or RNA template acceptor strands. Combined two-step clamp/DNA polymerase activity, where the initial clamp is followed by DNA synthesis, overview bovine immunodeficiency virus ?
-
?

Synonyms

Synonyms Comment Organism
reverse transcriptase
-
murine leukemia virus
reverse transcriptase
-
Human immunodeficiency virus 1
reverse transcriptase
-
bovine immunodeficiency virus

General Information

General Information Comment Organism
additional information the BIV reverse transcriptase is not stringent in the reaction parameters for clamp activity, such as the minimal complementarity length between the primer and functional template termini that sustains stable clamps, the effects of gaps between the two template strands on the clamp activity of the tested RTs, the effects of template end phosphorylations on the RT-associated clamp activities, and clamp activity with a long hairpin double-stranded primer comprising both the primer and the complementary non-functional template strands, overview. BIV is active with a single-nucleotide clamp substrate although its activity is substantially lower relative to the two-nucleotide clamp. The enzyme from BIV is able to stabilize even a single-nucleotide complementarity between the duplexed P/T2 bovine immunodeficiency virus
additional information the HIV-1 reverse transcriptase is stringent in the reaction parameters for clamp activity, such as the minimal complementarity length between the primer and functional template termini that sustains stable clamps, the effects of gaps between the two template strands on the clamp activity of the tested RTs, the effects of template end phosphorylations on the RT-associated clamp activities, and clamp activity with a long hairpin double-stranded primer comprising both the primer and the complementary non-functional template strands, overview. HIV-1 RT loses all apparent activity when tested with a single-nucleotide clamp substrate. The enzyme from HIV is not able to stabilize a single-nucleotide complementarity between the duplexed P/T2 Human immunodeficiency virus 1
additional information the MLV reverse transcriptase is stringent in the reaction parameters for clamp activity, such as the minimal complementarity length between the primer and functional template termini that sustains stable clamps, the effects of gaps between the two template strands on the clamp activity of the tested RTs, the effects of template end phosphorylations on the RT-associated clamp activities, and clamp activity with a long hairpin double-stranded primer comprising both the primer and the complementary non-functional template strands, overview. MLV RT loses all apparent activity when tested with s single-nucleotide clamp substrate. The enzyme from MIV is not able to stabilize a single-nucleotide complementarity between the duplexed P/T2 murine leukemia virus