Cloned (Comment) | Organism |
---|---|
expression of N-terminal His6-tagged p66/p66 homodimer HIV-1 RT in Escherichia coli strain BL21 (DE3) | Human immunodeficiency virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-deoxyadenosine | inhibits HIV-1 proviral DNA synthesis in human macrophages more efficiently than in CD4+ T cells | Human immunodeficiency virus 1 | |
additional information | ribonucleoside chain terminators may be a class of anti-HIV-1 agents specifically targeting viral macrophage infection | Human immunodeficiency virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis of HIV-1 RT rNTP incorporation | Human immunodeficiency virus 1 | |
0.0087 | - |
dATP | pH and temperature not specified in the publication | Human immunodeficiency virus 1 | |
0.059 | - |
dGTP | pH and temperature not specified in the publication | Human immunodeficiency virus 1 | |
0.155 | - |
dCTP | pH and temperature not specified in the publication | Human immunodeficiency virus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + DNAn | Human immunodeficiency virus 1 | - |
diphosphate + DNAn+1 | - |
? | |
dCTP + DNAn | Human immunodeficiency virus 1 | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Human immunodeficiency virus 1 | - |
diphosphate + DNAn+1 | - |
? | |
dGTP + DNAn | Human immunodeficiency virus 1 | - |
diphosphate + DNAn+1 | - |
? | |
additional information | Human immunodeficiency virus 1 | ribonucleoside triphosphate are efficiently incorporated into DNA in the macrophage but not in the T cell environment, detailed overview. HIV-1 RT initiates both (-)- and (+)-proviral DNA synthesis using RNA primers (e.g. tRNA3Lys and polypurine tract RNA primer) containing 3'-end ribonucleoside monophosphates during viral replication | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminal His6-tagged p66/p66 homodimer HIV-1 RT from Escherichia coli strain BL21 (DE3) by nickel affinity, anion exchange, and cation exchange chromatography | Human immunodeficiency virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + DNAn | - |
Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? | |
dCTP + DNAn | - |
Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Ext-T DNA 23-mer primer annealed to the RNA 40-mer template | Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? | |
dGTP + DNAn | - |
Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? | |
additional information | ribonucleoside triphosphate are efficiently incorporated into DNA in the macrophage but not in the T cell environment, detailed overview. HIV-1 RT initiates both (-)- and (+)-proviral DNA synthesis using RNA primers (e.g. tRNA3Lys and polypurine tract RNA primer) containing 3'-end ribonucleoside monophosphates during viral replication | Human immunodeficiency virus 1 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000083 | - |
dCTP | pH and temperature not specified in the publication | Human immunodeficiency virus 1 | |
0.00075 | - |
dATP | pH and temperature not specified in the publication | Human immunodeficiency virus 1 | |
0.00088 | - |
dGTP | pH and temperature not specified in the publication | Human immunodeficiency virus 1 |