Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.49 extracted from

  • Ndongwe, T.P.; Adedeji, A.O.; Michailidis, E.; Ong, Y.T.; Hachiya, A.; Marchand, B.; Ryan, E.M.; Rai, D.K.; Kirby, K.A.; Whatley, A.S.; Burke, D.H.; Johnson, M.; Ding, S.; Zheng, Y.M.; Liu, S.L.; Kodama, E.; Delviks-Frankenberry, K.A.; Pathak, V.K.; Mitsuya, H.; Parniak, M.A.; Singh, K.; Sarafianos, S.G.
    Biochemical, inhibition and inhibitor resistance studies of xenotropic murine leukemia virus-related virus reverse transcriptase (2012), Nucleic Acids Res., 40, 345-359.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged enzyme expression in Escherichia coli strain BL21-pLysS Moloney murine leukemia virus
His-tagged enzyme expression in Escherichia coli strain BL21-pLysS Xenotropic MuLV-related virus

Protein Variants

Protein Variants Comment Organism
K103R site-directed mutagenesis, the mutant is resistant to inhibition by tenofovir and 3'-azido-3'-deoxythymidine Moloney murine leukemia virus
K103R site-directed mutagenesis, the mutant is resistant to inhibition by tenofovir and 3'-azido-3'-deoxythymidine Xenotropic MuLV-related virus
Q190M site-directed mutagenesis, the mutant is resistant to inhibition by tenofovir and 3'-azido-3'-deoxythymidine Moloney murine leukemia virus
Q190M site-directed mutagenesis, the mutant is resistant to inhibition by tenofovir and 3'-azido-3'-deoxythymidine Xenotropic MuLV-related virus

Inhibitors

Inhibitors Comment Organism Structure
2',3'-dehydro-2',3'-deoxythymidine triphosphate i.e. stavudine triphosphate Moloney murine leukemia virus
2',3'-dehydro-2',3'-deoxythymidine triphosphate i.e. stavudine triphosphate Xenotropic MuLV-related virus
3'-azido-3'-deoxythymidine
-
Moloney murine leukemia virus
3'-azido-3'-deoxythymidine
-
Xenotropic MuLV-related virus
4'-ethynyl-2-amino-2'-deoxyadenosine triphosphate
-
Moloney murine leukemia virus
4'-ethynyl-2-amino-2'-deoxyadenosine triphosphate
-
Xenotropic MuLV-related virus
4'-ethynyl-2-fluoro-2'-deoxyadenosine triphosphate
-
Moloney murine leukemia virus
4'-ethynyl-2-fluoro-2'-deoxyadenosine triphosphate
-
Xenotropic MuLV-related virus
adefovir diphosphate
-
Moloney murine leukemia virus
adefovir diphosphate
-
Xenotropic MuLV-related virus
lamivudine triphosphate
-
Moloney murine leukemia virus
lamivudine triphosphate
-
Xenotropic MuLV-related virus
additional information the enzyme is highly susceptible to some nucleoside RT inhibitors, including translocation deficient RT inhibitors, but not to non-nucleoside RT inhibitors, e.g. TMC-125 and efavirenz, due to lack of two tyrosine residues involved in binding in enzymes from other virus Moloney murine leukemia virus
additional information the enzyme is highly susceptible to some nucleoside RT inhibitors, including translocation deficient RT inhibitors, but not to non-nucleoside RT inhibitors, e.g. TMC-125 and efavirenz, due to lack of two tyrosine residues involved in binding in enzymes from other virus Xenotropic MuLV-related virus
Tenofovir
-
Moloney murine leukemia virus
Tenofovir
-
Xenotropic MuLV-related virus
tenofovir diphosphate
-
Moloney murine leukemia virus
tenofovir diphosphate
-
Xenotropic MuLV-related virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics of nucleotide incorporation and DNA binding affinity using pre-steady state kinetics, DNA binding affinity using pre-steady state kinetics, overview Moloney murine leukemia virus
additional information
-
additional information steady-state kinetics of nucleotide incorporation and DNA binding affinity using pre-steady state kinetics, overview Xenotropic MuLV-related virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Moloney murine leukemia virus
Mg2+ required Xenotropic MuLV-related virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Moloney murine leukemia virus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Xenotropic MuLV-related virus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Moloney murine leukemia virus
-
-
-
Xenotropic MuLV-related virus A1Z651
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21-pLysS by nickel affinity chromatography and gel filtration Moloney murine leukemia virus
recombinant His-tagged enzyme from Escherichia coli strain BL21-pLysS by nickel affinity chromatography and gel filtration Xenotropic MuLV-related virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Moloney murine leukemia virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Xenotropic MuLV-related virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Td26/50-Cy3-Pd18b Moloney murine leukemia virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Td26/50-Cy3-Pd18b Xenotropic MuLV-related virus diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
Moloney murine leukemia virus RT
-
Moloney murine leukemia virus
MoMLV RT
-
Moloney murine leukemia virus
xenotropic murine leukemia virus-related virus reverse transcriptase
-
Xenotropic MuLV-related virus
XMRV RT
-
Xenotropic MuLV-related virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Moloney murine leukemia virus
37
-
assay at Xenotropic MuLV-related virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Moloney murine leukemia virus
7.8
-
assay at Xenotropic MuLV-related virus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00014
-
pH 7.8, 37°C Xenotropic MuLV-related virus 4'-ethynyl-2-amino-2'-deoxyadenosine triphosphate
0.00018
-
pH 7.8, 37°C Moloney murine leukemia virus 4'-ethynyl-2-amino-2'-deoxyadenosine triphosphate
0.00029
-
pH 7.8, 37°C Moloney murine leukemia virus 4'-ethynyl-2-fluoro-2'-deoxyadenosine triphosphate
0.00043
-
pH 7.8, 37°C Xenotropic MuLV-related virus 4'-ethynyl-2-fluoro-2'-deoxyadenosine triphosphate
0.00077
-
pH 7.8, 37°C Xenotropic MuLV-related virus 2',3'-dehydro-2',3'-deoxythymidine triphosphate
0.00092
-
pH 7.8, 37°C Xenotropic MuLV-related virus adefovir diphosphate
0.00102
-
pH 7.8, 37°C Moloney murine leukemia virus adefovir diphosphate
0.00151
-
pH 7.8, 37°C Moloney murine leukemia virus tenofovir diphosphate
0.00237
-
pH 7.8, 37°C Moloney murine leukemia virus 2',3'-dehydro-2',3'-deoxythymidine triphosphate
0.0064
-
pH 7.8, 37°C Xenotropic MuLV-related virus tenofovir diphosphate
0.01
-
pH 7.8, 37°C Moloney murine leukemia virus lamivudine triphosphate
0.021
-
pH 7.8, 37°C Xenotropic MuLV-related virus lamivudine triphosphate

General Information

General Information Comment Organism
malfunction kinetics of mismatch incorporation, overview Moloney murine leukemia virus
malfunction kinetics of mismatch incorporation, overview Xenotropic MuLV-related virus
additional information specifically the active site aspartates in motifs A and C, D150, D224, D225 in MoMLV RT, are conserved in the RT Moloney murine leukemia virus
additional information the enzyme is less efficient in DNA synthesis and in unblocking chain-terminated primers but has higher fidelity. Specifically the active site aspartates in motifs A and C, D150, D224, D225 in XMRV RT are conserved in the RT, molecular model of XMRV RT, overview Xenotropic MuLV-related virus