Cloned (Comment) | Organism |
---|---|
His-tagged enzyme expression in Escherichia coli strain BL21-pLysS | Moloney murine leukemia virus |
His-tagged enzyme expression in Escherichia coli strain BL21-pLysS | Xenotropic MuLV-related virus |
Protein Variants | Comment | Organism |
---|---|---|
K103R | site-directed mutagenesis, the mutant is resistant to inhibition by tenofovir and 3'-azido-3'-deoxythymidine | Moloney murine leukemia virus |
K103R | site-directed mutagenesis, the mutant is resistant to inhibition by tenofovir and 3'-azido-3'-deoxythymidine | Xenotropic MuLV-related virus |
Q190M | site-directed mutagenesis, the mutant is resistant to inhibition by tenofovir and 3'-azido-3'-deoxythymidine | Moloney murine leukemia virus |
Q190M | site-directed mutagenesis, the mutant is resistant to inhibition by tenofovir and 3'-azido-3'-deoxythymidine | Xenotropic MuLV-related virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',3'-dehydro-2',3'-deoxythymidine triphosphate | i.e. stavudine triphosphate | Moloney murine leukemia virus | |
2',3'-dehydro-2',3'-deoxythymidine triphosphate | i.e. stavudine triphosphate | Xenotropic MuLV-related virus | |
3'-azido-3'-deoxythymidine | - |
Moloney murine leukemia virus | |
3'-azido-3'-deoxythymidine | - |
Xenotropic MuLV-related virus | |
4'-ethynyl-2-amino-2'-deoxyadenosine triphosphate | - |
Moloney murine leukemia virus | |
4'-ethynyl-2-amino-2'-deoxyadenosine triphosphate | - |
Xenotropic MuLV-related virus | |
4'-ethynyl-2-fluoro-2'-deoxyadenosine triphosphate | - |
Moloney murine leukemia virus | |
4'-ethynyl-2-fluoro-2'-deoxyadenosine triphosphate | - |
Xenotropic MuLV-related virus | |
adefovir diphosphate | - |
Moloney murine leukemia virus | |
adefovir diphosphate | - |
Xenotropic MuLV-related virus | |
lamivudine triphosphate | - |
Moloney murine leukemia virus | |
lamivudine triphosphate | - |
Xenotropic MuLV-related virus | |
additional information | the enzyme is highly susceptible to some nucleoside RT inhibitors, including translocation deficient RT inhibitors, but not to non-nucleoside RT inhibitors, e.g. TMC-125 and efavirenz, due to lack of two tyrosine residues involved in binding in enzymes from other virus | Moloney murine leukemia virus | |
additional information | the enzyme is highly susceptible to some nucleoside RT inhibitors, including translocation deficient RT inhibitors, but not to non-nucleoside RT inhibitors, e.g. TMC-125 and efavirenz, due to lack of two tyrosine residues involved in binding in enzymes from other virus | Xenotropic MuLV-related virus | |
Tenofovir | - |
Moloney murine leukemia virus | |
Tenofovir | - |
Xenotropic MuLV-related virus | |
tenofovir diphosphate | - |
Moloney murine leukemia virus | |
tenofovir diphosphate | - |
Xenotropic MuLV-related virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of nucleotide incorporation and DNA binding affinity using pre-steady state kinetics, DNA binding affinity using pre-steady state kinetics, overview | Moloney murine leukemia virus | |
additional information | - |
additional information | steady-state kinetics of nucleotide incorporation and DNA binding affinity using pre-steady state kinetics, overview | Xenotropic MuLV-related virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Moloney murine leukemia virus | |
Mg2+ | required | Xenotropic MuLV-related virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Moloney murine leukemia virus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Xenotropic MuLV-related virus | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moloney murine leukemia virus | - |
- |
- |
Xenotropic MuLV-related virus | A1Z651 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21-pLysS by nickel affinity chromatography and gel filtration | Moloney murine leukemia virus |
recombinant His-tagged enzyme from Escherichia coli strain BL21-pLysS by nickel affinity chromatography and gel filtration | Xenotropic MuLV-related virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Moloney murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Xenotropic MuLV-related virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Td26/50-Cy3-Pd18b | Moloney murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Td26/50-Cy3-Pd18b | Xenotropic MuLV-related virus | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Moloney murine leukemia virus RT | - |
Moloney murine leukemia virus |
MoMLV RT | - |
Moloney murine leukemia virus |
xenotropic murine leukemia virus-related virus reverse transcriptase | - |
Xenotropic MuLV-related virus |
XMRV RT | - |
Xenotropic MuLV-related virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Moloney murine leukemia virus |
37 | - |
assay at | Xenotropic MuLV-related virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Moloney murine leukemia virus |
7.8 | - |
assay at | Xenotropic MuLV-related virus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00014 | - |
pH 7.8, 37°C | Xenotropic MuLV-related virus | 4'-ethynyl-2-amino-2'-deoxyadenosine triphosphate | |
0.00018 | - |
pH 7.8, 37°C | Moloney murine leukemia virus | 4'-ethynyl-2-amino-2'-deoxyadenosine triphosphate | |
0.00029 | - |
pH 7.8, 37°C | Moloney murine leukemia virus | 4'-ethynyl-2-fluoro-2'-deoxyadenosine triphosphate | |
0.00043 | - |
pH 7.8, 37°C | Xenotropic MuLV-related virus | 4'-ethynyl-2-fluoro-2'-deoxyadenosine triphosphate | |
0.00077 | - |
pH 7.8, 37°C | Xenotropic MuLV-related virus | 2',3'-dehydro-2',3'-deoxythymidine triphosphate | |
0.00092 | - |
pH 7.8, 37°C | Xenotropic MuLV-related virus | adefovir diphosphate | |
0.00102 | - |
pH 7.8, 37°C | Moloney murine leukemia virus | adefovir diphosphate | |
0.00151 | - |
pH 7.8, 37°C | Moloney murine leukemia virus | tenofovir diphosphate | |
0.00237 | - |
pH 7.8, 37°C | Moloney murine leukemia virus | 2',3'-dehydro-2',3'-deoxythymidine triphosphate | |
0.0064 | - |
pH 7.8, 37°C | Xenotropic MuLV-related virus | tenofovir diphosphate | |
0.01 | - |
pH 7.8, 37°C | Moloney murine leukemia virus | lamivudine triphosphate | |
0.021 | - |
pH 7.8, 37°C | Xenotropic MuLV-related virus | lamivudine triphosphate |
General Information | Comment | Organism |
---|---|---|
malfunction | kinetics of mismatch incorporation, overview | Moloney murine leukemia virus |
malfunction | kinetics of mismatch incorporation, overview | Xenotropic MuLV-related virus |
additional information | specifically the active site aspartates in motifs A and C, D150, D224, D225 in MoMLV RT, are conserved in the RT | Moloney murine leukemia virus |
additional information | the enzyme is less efficient in DNA synthesis and in unblocking chain-terminated primers but has higher fidelity. Specifically the active site aspartates in motifs A and C, D150, D224, D225 in XMRV RT are conserved in the RT, molecular model of XMRV RT, overview | Xenotropic MuLV-related virus |