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Literature summary for 2.7.7.5 extracted from
- Species specific release of sulfate from adenylyl sulfate by ATP sulfurylase or ADP sulfurylase in the green sulfur bacteria Chlorobium limicola and Chlorobium vibrioforme (1987), Arch. Microbiol., 147, 406-410.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| very labile , 50% loss of activity by four times freeze and thaw | Prosthecochloris vibrioformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | above 50 mM | Prosthecochloris vibrioformis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
adenosine 5'-phosphosulfate | pH 8.0, 50 mM phosphate | Prosthecochloris vibrioformis | |
| 8 | - |
phosphate | pH 8, 1 mM adenosine 5'-phosphosulfate | Prosthecochloris vibrioformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
gel filtration | Prosthecochloris vibrioformis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphate + adenosine 5'-phosphosulfate | Prosthecochloris vibrioformis | - |
ADP + SO42- | - |
? | |
| phosphate + adenosine 5'-phosphosulfate | Prosthecochloris vibrioformis 8327 | - |
ADP + SO42- | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prosthecochloris vibrioformis | - |
- |
- |
| Prosthecochloris vibrioformis 8327 | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C stable for several weeks | Prosthecochloris vibrioformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphate + adenosine 5'-phosphosulfate | - |
Prosthecochloris vibrioformis | ADP + SO42- | - |
? | |
| phosphate + adenosine 5'-phosphosulfate | - |
Prosthecochloris vibrioformis 8327 | ADP + SO42- | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
69% loss of activity within 5 min | Prosthecochloris vibrioformis |
| 70 | - |
no activity after 5 min | Prosthecochloris vibrioformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | slight differences in different buffer systems | Prosthecochloris vibrioformis |
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