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Literature summary for 2.7.7.50 extracted from

  • Itoh, N.; Mizumoto, K.; Kaziro, Y.
    Partial purification and characterization of mRNA guanylyltransferase from Saccharomyces cerevisiae (1983), FEBS Lett., 155, 161-166.
    View publication on PubMed
Search for more literature for 2.7.7.50

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Saccharomyces cerevisiae 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae
Mg2+ maximal activity at 0.5 mM Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 pep4, protease-deficient mutant
-

Purification (Commentary)

Purification (Comment) Organism
800fold Saccharomyces cerevisiae
partial Saccharomyces cerevisiae
physically associated with mRNA 5'-triphosphatase activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')RNA formation of a covalent intermediate enzyme-GMP Saccharomyces cerevisiae G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ir
additional information enzyme forms a covalent enzyme-GTP intermediate of apparent MW 45000 Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000-52000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Saccharomyces cerevisiae